Method for removing undifferentiated cells

A method for purifying differentiated cells from a population of differentiated and undifferentiated cells, wherein the method comprises:

• a) providing an input population of differentiated and undifferentiated cells to an inlet of a microfluidic device;
• b) flowing the input population of cells through at least one curvilinear microfluidic channel coupled to the inlet, wherein the curvilinear microfluidic channel is configured to separate differentiated cells from the input population of cells by size;
• and
• c) collecting a first output population of cells through one or more outlets coupled to the curvilinear microfluidic channel of the microfluidic device, wherein the first output population of cells has a higher proportion of differentiated cells relative to the input population of cells.

A device for removing undifferentiated cells from a population of differentiated and undifferentiated cells, the device comprising:

• a) an inlet for receiving an input population of differentiated and undifferentiated cells;
• b) at least one curvilinear microfluidic channel coupled to the inlet, wherein the curvilinear microfluidic channel is configured to separate undifferentiated cells from the input population of cells by size;
• and
• c) one or more outlets coupled to the curvilinear microfluidic channel for collecting an output population of cells, wherein the output population of cells has a lower proportion of undifferentiated cells relative to the input population of cells.

A device for purifying differentiated cells from a population of differentiated and undifferentiated cells, the device comprising:

• a) an inlet for receiving an input population of differentiated and undifferentiated cells;
• b) at least one curvilinear microfluidic channel coupled to the inlet, wherein the curvilinear microfluidic channel is configured to separate differentiated cells from the input population of cells by size;
• and
• c) one or more outlets coupled to the curvilinear microfluidic channel for collecting an output population of cells, wherein the output population of cells has a higher proportion of differentiated cells relative to the input population of cells.