Cleavable linkers for the tethering of polymerases to nucleotides
AssigneeAnsa Biotechnologies, Inc.
InventorsAaron Feldman, Sebastian Palluk, Daniel Arlow, Jeffrey George Bertram, Ben Raliski, Ronald T. Raines, Uwe Theo Bornscheuer, Nico Dennis Fessner, Christoffel Petrus Stephanus Badenhorst
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Abstract
The present disclosure provides methods of polynucleotide synthesis and compounds, compositions useful for synthesis of polynucleotides. The chemical compounds include nucleotides and their analogs that are attached to polymerase though a cleavable linker comprising an enzymatically cleavable amino acid ester.
Claims26
A conjugate comprising a polymerase, a nucleotide and a cleavable linker attached to the polymerase and the nucleotide, wherein the cleavable linker comprises an amino acid ester.
6-7. (canceled)
12-17. (canceled)
26-36. (canceled)
38-46. (canceled)
(canceled)
A modified nucleotide comprising a cleavable linker, wherein the cleavable linker comprises an amino acid ester.
50-81. (canceled)
Dependencies are inferred from the claim text.