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US 2026/0132162 A1Published application
Kits for improving the fluorescent signal of dmb-labeled sialic acids
AssigneeAgilent Technologies, Inc.
InventorFrancis T. HAXO
Life of this patent
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Abstract
The present invention provides methods and kits to improve the signal from sialic acids labeled with the dye 1, 2-diamino-4, 5-methylenedioxybenzene (“DMB”). The methods include labeling the sialic acids with DMB in an aqueous solution comprising the amino acid glycine, with pH adjusted to 1.5-3.2 by an acid, such as phosphoric acid or hydrochloric acid, and a reductant.
Claims15
1-68. (canceled)
A kit for labeling free sialic acids with 1, 2-diamino-4, 5-methylenedioxybenzene (“DMB”), said kit comprising (a) DMB, (b) glycine, (c) an acid, and (d) a reductant.
Dependencies are inferred from the claim text.