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US 2026/0126385 A1Published application
Methods and compositions for enhanced and selective photobleaching
AssigneeBruker Nano, Inc.
InventorsArne Christians, Thore Boettke, Jannik Boog, Oliver Braubach
Life of this patent
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Abstract
Disclosed herein are compositions, systems, kits, and methods for photobleaching, and selective photobleaching, a biological sample.
Claims30
A method for detection of one or more targets in a biological sample, the method comprising:
- a. contacting the biological sample with a detectable label and linking the detectable label to one or more targets;
- b. detecting a signal from the detectable label;
- c. after detection at step (b), contacting the sample of (b) with PMS, PDS, a salt thereof, or any combination thereof, wherein PMS and PDS are represented by the following formulas:
- d. irradiating the sample of (c), wherein irradiating comprises exposing the sample to light.
1 more element
A method for sequential detection of multiple targets in a biological sample, the method comprising:
- a. contacting a first target probe to the sample, wherein the first target probe is specific for a first target;
- b. hybridizing or binding the first target probe to the first target in the sample;
- c. detecting a first fluorescent detectable label, wherein the first fluorescent detectable label is linked to the first target probe;
- d. after detection at step (c), contacting the sample of (c) with PMS, PDS, a salt thereof, or any combination thereof, wherein PMS and PDS are represented by the following formulas:
- e. irradiating the sample of (d), wherein irradiating comprises exposing the sample to light;
- f. repeating steps (a)-(e), comprising an Nth target probe and an Nth fluorescent detectable label different from the first probe and first fluorescent detectable label.
3 more elements
A method for detecting a plurality of targets in a biological sample, the method comprising:
- a. contacting a plurality of target probes to the sample comprising N subsets of target probes, wherein each probe of the subsets of target probes is specific for different target;
- b. hybridizing or binding the target probes to the plurality of targets in the sample;
- c. detecting a first fluorescent detectable label, wherein the first fluorescent detectable label is linked to a first subset of the plurality of target probes;
- d. after detection at step (c), contacting the sample of (c) with PMS, PDS, a salt thereof, or any combination thereof, wherein PMS and PDS are represented by the following formulas:
- e. irradiating the sample of (d), wherein irradiating comprises exposing the sample to a light, optionally wherein the light is a selected wavelength range of light, wherein the selected wavelength range is within the wavelength range required to detect the first fluorescent detectable label;
- f. repeating steps (c)-(e), comprising an Nth fluorescent detectable label linked to an Nth probe, wherein the Nth fluorescent detectable label and the Nth subset of target probes are different from the first subset of target probe and the first fluorescent detectable label.
3 more elements
Dependencies are inferred from the claim text.