Production of molecules by periplasmic enzymatic catalysis

A process for producing a compound of interest from an organic substrate, comprising the steps of:

• a) preparing a reaction mixture comprising, in a suitable medium,
• n bacteria, n being an integer at least equal to 2, each bacterium being genetically modified to express in its periplasmic space at least one enzyme E1, E2, . . . En, each of said n bacteria expressing an enzyme different from the other bacteria,
• an enzyme E1 being able to catalyze a first reaction R1 from said organic substrate to provide a first product P1,
• each of said enzymes E2, . . . , En, being capable of catalyzing a reaction R2, . . . , Rn, from a product or a coproduct obtained by a reaction Rn−1, to provide, respectively, a product P2, . . . , Pn,
• said organic substrate,
• b) allowing the reaction mixture thus obtained to react, and
• c) separating the biomass from the supernatant and extracting therefrom said compound of interest consisting of one of the products P1, P2, . . . , Pn.