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US 2026/0125656 A1Published application
Baculovirus expression system
AssigneesCentre National De La Researche Scientifique (Cnrs), Institut National De La Researche Agronomique
InventorsMartine CERUTTI, Sylvie JULIANT, Sylvie THERY
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Abstract
The invention relates to a method for producing a recombinant baculovirus comprising n exogenous genes in an insect cell, by means of homologous recombination of a replication-deficient baculovirus genome and n transfer vectors, each comprising one of the n exogenous genes, n being an integer at least equal to 2.
Claims19
A recombinant baculovirus comprising n nucleotide sequences of formula (I):
- [exogenous gene]-[spacer nucleotide sequence]-[functional gene essential for viral replication] (I),
- said spacer nucleic acid sequence consists of 0 to 600 bp,
- said functional gene essential for viral replication is selected from 1629 (ORF9), Pk1 (ORF10), lef-1 (ORF14), ORF34, lef-11 (ORF37), p47 (ORF40), lef8 (ORF50), DNAJ domain (ORF51), ORF53, vp1054 (ORF54), Lef-9 (ORF62), DNA Pol (ORF65), lef-3 (ORF67), ORF73, ORF75, ORF81, p95 (ORF83), vp39 (ORF89), lef-4 (ORF90), p33 (ORF92), helicase (ORF95), Vp80 (ORF104), ORF106-107, odv-ec43 (ORF109), gp64/67 (ORF128), ORF132, ORF133, odv-ec27 (ORF144), ORF146, ie1 (ORF147), lef-2 (ORF6);
- n being an integer at least equal to 2,
- said recombinant baculovirus does not comprise a nucleic acid sequence allowing it to replicate within a bacterial cell.
2 more elements
A recombinant baculovirus comprising n nucleotide sequences of formula (I):
- [exogenous gene]-[spacer nucleotide sequence]-[functional gene essential for viral replication] (I),
- said spacer nucleic acid sequence consists of 0 to 600 bp,
- said functional gene essential for viral replication is selected from 1629 (ORF9), Pk1 (ORF10), lef-1 (ORF14), ORF34, lef-11 (ORF37), p47 (ORF40), lef8 (ORF50), DNAJ domain (ORF51), ORF53, vp1054 (ORF54), Lef-9 (ORF62), DNA Pol (ORF65), lef-3 (ORF67), ORF73, ORF75, ORF81, p95 (ORF83), vp39 (ORF89), lef-4 (ORF90), p33 (ORF92), helicase (ORF95), Vp80 (ORF104), ORF106-107, odv-ec43 (ORF109), gp64/67 (ORF128), ORF132, ORF133, odv-ec27 (ORF144), ORF146, ie1 (ORF147), lef-2 (ORF6);
- n being an integer at least equal to 2,
- said recombinant baculovirus does not comprise a nucleic acid sequence allowing it to replicate within a bacterial cell.
2 more elements
A set of homologous recombination elements comprising:
- a) a replication-deficient baculovirus genome in which n genes essential for viral replication are non-functional;
- b) n transfer vectors each comprising:
- i) a nucleic acid sequence that restores the function of one of the n non-functional genes essential for viral replication,
- ii) optionally an exogenous gene,
- n being an integer at least equal to 2.
2 more elements
Dependencies are inferred from the claim text.