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US 2026/0125635 A1Published application
New lactic acid bacteria
AssigneeInternational N&H Denmark Aps
InventorsAnais JEDRZEJOWSKI, Christophe FREMAUX, Sabine VAN DILLEN, Thomas DESFOUGERES, Max Charles JODEAU, Damien LUGAND
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Abstract
The invention relates to a polynucleotide comprising a lacZ gene (lacZFS) encoding a β-galactosidase characterized by a particular profile regarding its efficiency of hydrolysis of lactose. The invention is also directed to a Streptococcus thermophilus strain comprising a lacZFS allele and bacterial composition thereof, and their use to obtain fermented milk not undergoing post-acidification.
Claims9
A polynucleotide encoding a β-galactosidaseFS, which consist of an amino acid sequence identified by SEQ ID NO:4;
- and which is defined as a lacZ allele which increases the ratio of the activity of lactose importation of the LacS permease at pH 4.5 over the activity of lactose hydrolysis of the beta-galactosidase at pH 4.5 above 8 in a DGCC715 derivative, said DGCC715 derivative being a strain DGCC715 (deposited at the DSMZ on Feb. 12, 2019 under the accession number DSM33036), into which its lacZ gene was replaced by said polynucleotide encoding a β-galactosidaseFS.
Dependencies are inferred from the claim text.