Antigen chromatography columns and methods of use thereof

A method of loading a biotinylated antigen to a streptavidin column to form an antigen chromatography column, the method comprising:

• a) selecting a streptavidin column having a plurality of particles, each particle comprising:
• a nonporous polymer core;
• a hydrophilic surface on an outer layer of the nonporous polymer core;
• and
• one or more molecules of streptavidin conjugated to the hydrophilic surface, wherein the streptavidin has a plurality of accessible binding sites,
• wherein the particle has an average particle size between 1.0 μm to 10 μm;
• b) applying a solution of biotinylated antigens to the streptavidin column such that the biotinylated antigens bind to a portion of the plurality of accessible binding sites of streptavidin to form the antigen chromatography column.