Rhinovirus mrna vaccine
AssigneeSanofi Pasteur Limited
InventorsCatherine BERRY, Cyril CHAVAGNAC, Nicholas CLARK, Yves GIRERD-CHAMBAZ, Valerie LECOUTURIER, Nathalie MANTEL, Vincent PAVOT, Khang Anh TRAN
Life of this patent
Estimating the term…
Abstract
The present invention provides a method for identifying the amino acid sequence of a naturally occurring polyprotein from a group A or C rhinovirus that can be used as an immunogen capable of eliciting an immune response against rhinoviruses from multiple serotypes within the same group. The invention also provides immunogenic compositions that comprise at least one mRNA comprising a non-naturally occurring optimized nucleic acid encoding a polyprotein identified by this method.
Claims42
A method of identifying a rhinovirus polyprotein for use as an immunogen that is capable of eliciting an immune response against rhinoviruses from multiple serotypes within a group, said method comprising:
- (a) retrieving a plurality of amino acid sequences from a database comprising amino acid sequences from naturally occurring rhinovirus isolates;
- (b) removing from the plurality of amino acid sequences retrieved in step (a) amino acid sequences shorter than 800 amino acids;
- (c) assigning the amino acid sequences remaining after step (b) into different phylogenetic clusters;
- (d) aligning the amino acid sequences to determine a consensus amino acid sequence for a complete rhinovirus polyprotein for one or more phylogenetic clusters identified in step (c);
- (e) aligning the consensus amino acid sequence obtained in step (d) with complete polyproteins of naturally occurring rhinovirus isolates;
- and
- (f) selecting a rhinovirus polyprotein as an immunogen that has an average identity of at least 80% to the corresponding amino acid sequences of rhinoviruses from at least two phylogenetic clusters identified in step (c).
3-8. (canceled)
10-11. (canceled)
An immunogenic composition comprising at least one messenger RNA (mRNA) comprising a first non-naturally occurring optimized nucleic acid sequence encoding a first polyprotein from a group A or C rhinovirus, wherein said first polyprotein has an amino acid sequence that:
- (a) has an average identity of at least 80% to the amino acid sequences of corresponding polyproteins from at least two, at least three, or at least four phylogenetic clusters of rhinoviruses of the same group;
- and
- (b) is naturally occurring aside from an optional single amino acid substitution.
(canceled)
16-25. (canceled)
(canceled)
30-39. (canceled)
(canceled)
43-46. (canceled)
48-58. (canceled)
60-72. (canceled)
75-85. (canceled)
(canceled)
89-92. (canceled)
94-96. (canceled)
99-104. (canceled)
106-110. (canceled)
113-126. (canceled)
128-129. (canceled)
Dependencies are inferred from the claim text.