Staphylococcus phage compositions and cocktails thereof

A composition comprising a plurality of bacteriophage comprising a first bacteriophage and a second bacteriophage, wherein a bacteria treated with the plurality of bacteriophage has a reduced amount of regrowth as compared to treatment with the first bacteriophage or the second bacteriophage alone, wherein the first bacteriophage comprises a Rosenblumvirus and the second bacteriophage comprises a Phietavirus or a Kayvirus.

A bacteriophage engineered to render the bacteriophage lytic by removal, replacement, or inactivation of a lysogenic sequence, wherein the lysogenic sequence comprises at least 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, or 100% of a sequence selected from SEQ ID NOS:

• 49-58.

A bacteriophage comprising at least 70%, 75%, 80%, 85%, 90%, 95%, or 100% sequence identity with p1378 (PTA-127329).

A bacteriophage comprising at least 70%, 75%, 80%, 85%, 90%, 95%, or 100% sequence identity with p4815 (PTA-127331).

A bacteriophage comprising at least 70%, 75%, 80%, 85%, 90%, 95%, or 100% sequence identity with p1494e002 (PTA-127345).

A bacteriophage comprising at least 70%, 75%, 80%, 85%, 90%, 95%, or 100% sequence identity with p2808 (PTA-127332).

A composition comprising at least two bacteriophage, wherein a first bacteriophage comprises at least 70%, 75%, 80%, 85%, 90%, 95%, or 100% sequence identity with p1378, and a second bacteriophage comprises at least 70%, 75%, 80%, 85%, 90%, 95%, or 100% sequence identity with p1381, p4815, p1378e062, p1378e074, p4815e037, p1378e075, p1381e017, p4815e053, p1498e001, p3693e001, p3224e002, p5593e001, p1468e003, p1478e003, p1494e002, p2808, or p1473.

A composition comprising at least two bacteriophage, wherein a first bacteriophage comprises at least 70%, 75%, 80%, 85%, 90%, 95%, or 100% sequence identity with p4815, and a second bacteriophage comprises at least 70%, 75%, 80%, 85%, 90%, 95%, or 100% sequence identity with p1381, p1378, p1378e062, p1378e074, p4815e037, p1378e075, p1381e017, p4815e053, p1498e001, p3693e001, p3224e002, p5593e001, p1468e003, p1478e003, p1494e002, p2808, or p1473.

A composition comprising at least two bacteriophage, wherein a first bacteriophage comprises at least 70%, 75%, 80%, 85%, 90%, 95%, or 100% sequence identity with p1494e002, and a second bacteriophage comprises at least 70%, 75%, 80%, 85%, 90%, 95%, or 100% sequence identity with p1381, p1378, p4815, p1378e062, p1378e074, p4815e037, p1378e075, p1381e017, p4815e053, p1498e001, p3693e001, p3224e002, p5593e001, p1468e003, p1478e003, p2808, or p1473.

A composition comprising at least two bacteriophage, wherein a first bacteriophage comprises at least 70%, 75%, 80%, 85%, 90%, 95%, or 100% sequence identity with p2808, and a second bacteriophage comprises at least 70%, 75%, 80%, 85%, 90%, 95%, or 100% sequence identity with p1381, p1378, p4815, p1378e062, p1378e074, p4815e037, p1378e075, p1381e017, p4815e053, p1498e001, p3693e001, p3224e002, p5593e001, p1468e003, p1478e003, p1494e002, or p1473.

The composition of any one of claims 33-38, wherein the collection of Staphylococcus bacteria comprises comprise Staphylococcus bacteria having a MLST of 8, 5, 22, 15, 1, 30, 398, 105, 45, 672, 2250, 582, 72, 97, 239, 34, 87, 101, 109, 1159, 1165, 1181, 12, 121, 152, 1750, 20, 225, 25, 291, 3628, 59, 7, 779, 88, 10, 1011, 1049, 1156, 1351, 149, 1637, 1649, 1757, 1842, 188, 1970, 2066, 256, 2867, 2945, 3149, 3182, 3510, 39, 395, 4317, 47, 4730, 50, 508, 573, 6, 630, 737, 828, 848, 923, or 93, or a combination of two or more thereof.