Adhesion molecule inhibition for stem cell therapies
AssigneeWisconsin Alumni Research Foundation
InventorMatthew Brown
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Abstract
An in vitro method of preparing a population of hypoimmune mammalian stem cells includes providing a population of isolated mammalian stem cells, wherein the isolated mammalian stem cells express a cell adhesion molecule; and modifying the expression of the cell adhesion molecule in the population of isolated mammalian stem cells to decrease or knockout expression of the cell adhesion molecule and provide the population of hypoimmune mammalian cells. The population of isolated mammalian stem cells can be pluripotent stem cells, or embryonic stem cells, and can be human or non-human stem cells.
Claims24
An in vitro method of preparing a population of hypoimmune mammalian stem cells, comprising:
- providing a population of isolated mammalian stem cells, wherein the isolated mammalian stem cells express a cell adhesion molecule;
- and
- modifying the expression of the cell adhesion molecule in the population of isolated mammalian stem cells to decrease or knockout expression of the cell adhesion molecule and provide the population of hypoimmune mammalian stem cells.
A population of hypoimmune mammalian stem cells, wherein expression of a cell adhesion molecule is decreased or knocked out by modification by gene editing of a gene for the cell adhesion molecule.
A graft comprising the population of differentiated hypoimmune mammalian stem cells of claim 14.
Dependencies are inferred from the claim text.