Methanotroph strains for mitigating methane and methods related thereto
AssigneeNewLeaf Symbiotics, Inc.
InventorsNatalie Breakfield, Desmond R. Jimenez, David Flack, Anthony Neumann
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Abstract
Methanotroph strains that enhance early growth of plants, improve propagation/transplant vigor, increase nutrient uptake, improve stand establishment, improve stress tolerance and/or increase a plant's ability to utilize nutrients are provided herein. Uses of compostions comprising such strains and optionally methylotroph strains, for methane mitigation and crop improvement is provided. Also provided are methods to reduce green-house gas emission and convert methane to methanol with methanotroph strains.
Claims33
A method for mitigating methane gas in an agricultural field that comprises:
- (a) applying a composition to a soil, field, plant, plant part or seed, wherein the composition comprises at least one methanotroph selected from the group consisting of a Type II methanotroph comprising a pMMO2 protein, and Type I methanotroph,
- (b) growing the methanotroph whereby the methanotroph uses methane as a carbon source;
- wherein use of the methane as the carbon source oxidizes methane or reduces methane emissions.
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A composition comprising a fermentation product comprising a methanotroph strain, wherein said fermentation product is essentially free of contaminating microorganisms, and wherein methanotroph strain comprises NLS1501 (NRRL B-68261), NLS1504 (NRRL B-68281), NLS1505 (NRRL B-68282), NLS1506 (NRRL B-68283), NLS1508 (NRRL B-68262), NLS1509 (NRRL B-68284), NLS1511 (NRRL B-68285), NLS1512 (NRRL B-68286), or a variant thereof.
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A method for mitigating methanol that comprises:
- (a) treating a rice field with a composition comprising at least one methanotroph selected from the group consisting of a Type II methanotroph comprising a pMMO2 protein, and Type I methanotroph, wherein said Type I methanotroph is a Methylomicrobium sp. or a Methylosarcina sp.;
- and
- (b) growing the methanotroph in the field thereby mitigating methane.
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An isolated methanotroph selected from NLS1501 (NRRL B-68261), NLS1504 (NRRL B-68281), NLS1505 (NRRL B-68282), NLS1506 (NRRL B-68283), NLS1508 (NRRL B-68262), NLS1509 (NRRL B-68284), NLS1511 (NRRL B-68285), NLS1512 (NRRL B-68286).
A method for selecting a methanotroph isolate capable of utilizing methane as a food source, wherein said method comprises:
- a) detecting in the genome of a methanotroph isolate, one or more genetic elements, wherein said genetic element comprises a particulate methane monooxygenase;
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- b) treating a field, water, plant, plant part or seed with said methanotroph isolate, and measuring green-house gas emissions.
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A method for increasing harvested yield of a plant, wherein said method comprises applying a composition to a soil, field, plant, plant part or seed, wherein the composition comprises at least one methanotroph.
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Dependencies are inferred from the claim text.