Controller for a laser microdissection system, laser microdissection system, and method for laser microdissection

A controller for a laser microdissection system, wherein the controller is configured to:

• receive a first image of a sample comprising multiple image tiles stitched together, and a first set of coordinates in a coordinate system of the first image, the first set of coordinates defining at least one segment to be removed from the sample;
• control the laser microdissection system to generate at least one second image of a part of the sample;
• generate at least one second set of coordinates in a coordinate system of the second image, each of the second sets of coordinates corresponding to at least a subset of the first set of coordinates;
• control the laser microdissection system based on the at least one second set of coordinates to remove the at least one segment from the sample;
• determine at least two reference points on the sample visible in the first image and the at least one second image;
• determine original coordinates corresponding to coordinates of the reference points in the coordinate system of the first image;
• determine target coordinates corresponding to coordinates of the reference points in the coordinate system of the at least one second image;
• and
• generate the at least one second set of coordinates based on the original coordinates and the target coordinates.