Methods and apparatus for hemolysis detection
AssigneeSiemens Healthcare Diagnostics Inc.
InventorJeffery Franklin
Life of this patent
Estimating the term…
Abstract
A sensor assembly enabling hemolysis detection with a diagnostic analyzer. The sensor assembly can be included in a diagnostic cartridge that may be coupled to the diagnostic analyzer and a sample fluid can be delivered thereto. The sensor assembly includes a main oxygen sensor configured to provide a first measurement and a modified oxygen sensor including an oxidant configured to oxidize hemoglobin iron to methemoglobin and configured to provide a second measurement. A hemolysis detection module of the diagnostic analyzer provides a level of hemolysis in the sample based on a difference between the first measurement and the second measurement. Numerous other diagnostic analyzers, diagnostic cartridges, and detection methods are provided.
Claims16
A diagnostic sensor assembly, comprising:
- a sample inlet configured to receive a sample;
- a sample passageway extending from the sample inlet;
- a main oxygen sensor configured to contact the sample along the sample passageway;
- and
- a modified oxygen sensor configured to contact the sample along the sample passageway, the modified oxygen sensor comprising an oxidant configured to oxidize hemoglobin to methemoglobin, from which quantification of hemolysis of the sample can be obtained.
A diagnostic analyzer, comprising:
- an analyzer body including a cartridge receiver and an electrical connector;
- a diagnostic cartridge receivable in the cartridge receiver, the diagnostic cartridge comprising:
- a cartridge body configured to be coupled to the cartridge receiver, the cartridge body including:
- electrical contacts couplable to the electrical connector when the cartridge body is received into the cartridge receiver,
- a sample inlet configured to receive a sample;
- a sample passageway extending into the cartridge body from the sample inlet and configured to receive the sample therein;
- a main oxygen sensor configured to contact the sample in the sample passageway and configured to provide a first measurement of the sample;
- a modified oxygen sensor configured to contact the sample in the sample passageway, the modified oxygen sensor including an oxidant configured to oxidize hemoglobin to methemoglobin and configured to provide a second measurement of the sample;
- and
- a controller coupled to the electrical connector, the controller configured to receive the first measurement and the second measurement of the sample and configured to provide a level of hemolysis in the sample based on the first measurement and the second measurement.
A method of determining hemolysis of a sample, comprising:
- coupling a diagnostic cartridge to a cartridge receiver of a diagnostic analyzer, the diagnostic cartridge comprising a sample inlet configured to receive the sample, a sample passageway extending into a cartridge body from the sample inlet, a main oxygen sensor configured to contact the sample in the sample passageway, and a modified oxygen sensor configured to contact the sample in the sample passageway, the modified oxygen sensor including an oxidant configured to oxidize extracellular hemoglobin to methemoglobin;
- passing the sample through the sample passageway and into contact with the main oxygen sensor and the modified oxygen sensor;
- obtaining a first measurement of the sample from the main oxygen sensor;
- obtaining a second measurement of the sample from the modified oxygen sensor;
- and
- providing a level of hemolysis in the sample based on the first measurement and the second measurement.
Dependencies are inferred from the claim text.