Method for determining multi-characteristic components in anshen dingzhi wan

Abstract

A method for determining multi-characteristic components of ASDZW is provided. By optimizing the gradient elution program of high performance liquid chromatography (HPLC), strictly controlling the initial mobile phase ratio of the gradient elution program, cooperating with the selections of the chromatographic column and the mobile phase, the invention finally realizes the simultaneous, rapid and comprehensive determination of six characteristic components in ASDZW under the condition of a single wavelength; specifically, the six characteristic components are polygalaxanthone III, 3,6′-disinapoyl sucrose, ginseng saponin Rb1, β-asarone, dehydrotumulosic acid, and pachymic acid. The whole detection process is a single wavelength detection with high detection efficiency and short analysis time, and the detection limits of the six components are lower than 0.008 μg/mL, meaning this method has high sensitivity, good specificity, accurate results, and good reproducibility.

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