Systems and methods for robotic capillary microsampling

A method of microsampling, comprising:

• moving a sample holder including at least a first sample volume within a sealed internal environment such that a capillary disposed within the sealed internal environment is operably positioned relative to the first sample volume;
• pressurizing the sealed internal environment to inject at least a portion of the first sample volume into the capillary;
• moving the sample holder such that the capillary, including the at least the portion of the first sample volume, is operably positioned relative to a background electrolyte electrically coupled to a voltage source;
• and
• applying electrical energy from the voltage source to separate the at least the portion of the first sample volume within the capillary via capillary electrophoresis.

A non-transitory computer-readable storage medium storing instructions that, when executed by at least one processor, cause the at least one processor to control:

• movement of a sample holder including at least a first sample volume within a sealed internal environment such that a capillary disposed within the sealed internal environment is operably positioned relative to the first sample volume;
• pressurization of the sealed internal environment to inject at least a portion of the first sample volume into the capillary;
• movement of the sample holder such that the capillary, including the at least the portion of the first sample volume, is operably positioned relative to a background electrolyte electrically coupled to a voltage source;
• and
• application of electrical energy from the voltage source to separate the at least the portion of the first sample volume within the capillary via capillary electrophoresis.