Genetically engineered microbe for production of isoprene from a carbon source and method of production thereof

A method of producing isoprene from carbon source, the method comprising:

• culturing a genetically engineered microbe under suitable conditions, the genetically engineered microbe comprising:
• a first nucleic acid sequence encoding a first enzyme, wherein the first enzyme is configured to catalyze one or more steps of a conversion from ethanol to acetyl coenzyme A-CoA;
• a second nucleic acid sequence encoding a second enzyme of an MVA pathway;
• and
• a heterologous nucleic acid sequence encoding a third enzyme, wherein the third enzyme is configured to catalyze an isoprene-producing chemical reaction;
• and
• providing a substrate to the cultured genetically engineered microbe.