Genetically engineered peptide superstructure and microbe for production of isoprene and method of production thereof
AssigneeCleanJoule, Inc.
InventorsJonathan Cloyd Greenhalgh, Robert Andrew Price
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Abstract
A genetically engineered microbe capable of producing isoprene, wherein the genetically engineered microbe including a heterologous nucleic acid construct encoding at least a portion of a peptide superstructure; the peptide superstructure including a first portion including a first binding site configured to catalyze a first chemical reaction, wherein the first reaction converts a substrate to an intermediate; and a second portion including a second binding site configured to catalyze a second chemical reaction, wherein the second binding site is located in proximity to the first binding site and the second chemical reaction converts the intermediate to isoprene.
Claims20
A genetically engineered microbe capable of producing isoprene, the genetically engineered microbe comprises:
- a heterologous nucleic acid construct encoding at least a portion of a peptide superstructure, wherein the superstructure comprises:
- a first portion comprising a first binding site configured to catalyze a first chemical reaction, wherein the first reaction converts a substrate to an intermediate;
- and
- a second portion comprising a second binding site configured to catalyze a second chemical reaction, wherein:
- the second binding site is located in proximity to the first binding site;
- and
- the second chemical reaction converts the intermediate to isoprene.
A genetically engineered peptide superstructure for production of isoprene, the superstructure comprising:
- a first portion comprising at least a functional fragment of isoprene synthase (IspS) configured to catalyze a first chemical reaction;
- and
- a second portion comprising at least a functional fragment of isopentenyl diphosphate (IPP) isomerase configured to catalyze a second chemical reaction, wherein the second chemical reaction converts a substrate to an intermediate, and wherein:
- the second portion is covalently linked to the first portion through a linker;
- and
- the intermediate is converted into isoprene by the first chemical reaction.
A genetically engineered peptide superstructure for production of isoprene, the superstructure comprising:
- a first portion comprising at least a functional fragment of isopentenyl diphosphate (IPP) isomerase configured to catalyze a first chemical reaction, wherein the first chemical reaction converts a substrate to an intermediate;
- and
- a second portion comprising at least a functional fragment of isoprene synthase (IspS) configured to catalyze a second chemical reaction, wherein:
- the second portion is covalently linked to the first portion through a linker;
- and
- the intermediate is converted into isoprene by the second chemical reaction.
A method of producing isoprene using a genetically engineered peptide superstructure, the method comprising:
- culturing a genetically engineered microbe under suitable conditions, wherein:
- the genetically engineered microbe comprises a heterologous nucleic acid construct encoding at least a portion of a peptide superstructure;
- and
- the superstructure comprises:
- a first portion comprising a first binding site;
- and
- a second portion comprising a second binding site, the second portion is covalently linked to the first portion through a linker;
- providing a substrate to the cultured genetically engineered microbe, wherein:
- the first binding site is configured to bind to the substrate and catalyze a first chemical reaction that converts the substrate to an intermediate;
- and
- the second binding site is configured to catalyze a second chemical reaction that converts the intermediate to isoprene.
Dependencies are inferred from the claim text.