Genetically engineered microbe for the production of terpenes and method of production thereof
AssigneeCleanJoule, Inc.
InventorsYounghwan Kim, Bohea Lee, Robert Andrew Price
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Abstract
A genetically engineered microbe capable of producing isoprene from glycerol includes at least a native nucleic acid sequence encoding at least a native enzyme capable of catalyzing one or more steps of a conversion from at least a carbon source to acetyl coenzyme A (A-CoA), at least a first heterologous nucleic acid sequence encoding at least a first enzyme of a mevalonate (MVA) pathway, and at least a second heterologous nucleic acid sequence encoding at least a second enzyme capable of catalyzing at least a terpene-producing chemical reaction, wherein the at least a carbon source includes glycerol.
Claims26
A genetically engineered microbe capable of producing a terpene, the genetically engineered microbe comprising:
- at least a native nucleic acid sequence encoding at least a native enzyme capable of catalyzing one or more steps of a conversion of a carbon source to acetyl coenzyme A (A-CoA), wherein the carbon source comprises glycerol, wherein the conversion includes one or more steps utilizing glycerol as a substrate, wherein the genetically engineered microbe comprises a genetically engineered bacterium, and wherein the genetically engineered bacterium comprises at least Vibrio natriegens;
- at least a first heterologous nucleic acid sequence encoding at least a first enzyme of a mevalonate (MVA) pathway;
- and
- at least a second heterologous nucleic acid sequence encoding at least a second enzyme capable of catalyzing at least a terpene-producing chemical reaction.
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A method of producing a terpene, the method comprising:
- culturing a genetically engineered microbe under suitable conditions, the genetically engineered microbe comprising:
- at least a native nucleic acid sequence encoding at least a native enzyme capable of catalyzing one or more steps of a conversion of a carbon source to acetyl coenzyme A (A-CoA), wherein the carbon source comprises glycerol, wherein the genetically engineered microbe comprises a genetically engineered bacterium, and wherein the genetically engineered bacterium comprises at least Vibrio natriegens;
- at least a first heterologous nucleic acid sequence encoding at least a first enzyme of a mevalonate (MVA) pathway;
- and
- at least a second heterologous nucleic acid sequence encoding at least a second enzyme capable of catalyzing at least a terpene-producing chemical reaction;
- and
- providing a substrate to the cultured genetically engineered microbe, wherein the conversion includes one or more steps utilizing glycerol as the substrate.
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Dependencies are inferred from the claim text.