cis AND trans REQUIREMENTS FOR TERMINAL RESOLUTION OF HUMAN BOCAVIRUS 1
AssigneesUniversity of Iowa Resarch Foundation, University of Kansas
InventorsJohn F. Engelhardt, Ziying Yan, Jianming Qiu
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Abstract
A recombinant mutant BoV genome is provided, as well as methods of using the vector, e.g., to prepare helper-free virus.
Claims17
(canceled)
A method to express a transgene in a cell comprising introducing a composition comprising a recombinant parvovirus vector comprising a synthetic enhancer comprising a plurality of enhancer sequences operably linked to a promoter operably linked to a transgene to a eukaryotic cell so as to express the transgene in the cell.
A method to express a human CFTRAR transgene in a cell comprising introducing a composition comprising a recombinant parvovirus vector to a eukaryotic cell so as to express the human CFTRAR transgene in the cell, wherein the recombinant parvovirus vector comprises (i) an enhancer operably linked to a promoter, wherein the enhancer and promoter comprise the sequence of SEQ ID NO:
- 27, and (ii) the human CFTRΔR transgene operably linked to the promoter.
Dependencies are inferred from the claim text.