Plants Having Increased Tolerance to Herbicides
AssigneeBASF Agro B.V.
InventorsRaphael Aponte, Stefan Tresch, Matthias Witschel, Jens Lerchl, Jill Marie Paulik, Chad Brommer
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Abstract
The present invention refers to a method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding a wild-type or a mutated protoporphyrinogen oxidase (PPO) which is resistant or tolerant to a benzoxazinone-derivative herbicide by applying to said site an effective amount of said herbicide. The invention further refers to plants comprising wild-type or mutated PPO enzymes, and methods of obtaining such plants.
Claims23
A method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of:
- a) providing, at said site, a plant that comprises at least one nucleic acid comprising
- a nucleotide sequence encoding a wild-type protoporphyrinogen oxidase or a mutated protoporphyrinogen oxidase (mut-PPO) which is resistant or tolerant to a benzoxazinone-derivative herbicide and/or
- b) applying to said site an effective amount of said herbicide.
A method for identifying a benzoxazinone-derivative herbicide by using a mut-PPO encoded by a nucleic acid which comprises the nucleotide sequence of SEQ ID NO:
- 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, or 45, or a variant or derivative thereof.
A method of identifying a nucleotide sequence encoding a mut-PPO which is resistant or tolerant to a benzoxazinone-derivative herbicide, the method comprising:
- a) generating a library of mut-PPO-encoding nucleic acids,
- b) screening a population of the resulting mut-PPO-encoding nucleic acids by expressing each of said nucleic acids in a cell or plant and treating said cell or plant with a benzoxazinone-derivative
- c) comparing the benzoxazinone-derivative tolerance levels provided by said population of mut-PPO encoding nucleic acids with the benzoxazinone-derivative tolerance level provided by a control PPO-encoding nucleic acid,
- d) selecting at least one mut-PPO-encoding nucleic acid that provides a significantly increased level of tolerance to a benzoxazinone-derivative as compared to that provided by the control PPO-encoding nucleic acid.
A mut-PPO polypeptide comprising a sequence of SEQ ID NO:
- 2 or SEQ ID NO:4, a variant, derivative, othologue, paralogue or homologue thereof, in which the amino acid at position 420 is Met or Val, and the the amino acid at position 397 is Gly, Ala, Ser, Thr, Cys, Val, Ile, Met, Pro, Phe, Tyr, Trp, His, Lys, Arg, Asn, Asp, Glu, or Gin, wherein said mut-PPO polypeptide confers increased resistance or tolerance to a benzoxazinone-derivative herbicide in a plant as compared to a wild type plant.
A transgenic plant cell transformed by a nucleic acid encoding a mut-PPO polypeptide, wherein expression of the nucleic acid in the plant cell results in increased resistance or tolerance to a benzoxazinone-derivative herbicide as compared to a wild type variety of the plant cell.
A plant that expresses a mutagenized or recombinant mut-PPO polypeptide comprising the sequence of SEQ ID NO:
- 2 or SEQ ID NO:4, a variant, derivative, othologue, paralogue or homologue thereof, in which the amino acid at position 420 is Met or Val, and the the amino acid at position 397 is Gly, Ala, Ser, Thr, Cys, Val, Ile, Met, Pro, Phe, Tyr, Trp, His, Lys, Arg, Asn, Asp, Glu, or Gin, and wherein said mut-PPO confers upon the plant increased herbicide tolerance as compared to the corresponding wild-type variety of the plant when expressed therein.
A method of producing a transgenic plant cell with an increased resistance to a benzoxazinone-derivative herbicide as compared to a wild type variety of the plant cell comprising, transforming the plant cell with an expression cassette comprising a nucleic acid encoding a mut-PPO polypeptide.
A method of producing a transgenic plant comprising, (a) transforming a plant cell with an expression cassette comprising a nucleic acid encoding a mut-PPO polypeptide, and (b) generating a plant with an increased resistance to benzoxazinone-derivative herbicide from the plant cell.
A method of identifying or selecting a transformed plant cell, plant tissue, plant or part thereof comprising:
- i) providing a transformed plant cell, plant tissue, plant or part thereof, wherein said transformed plant cell, plant tissue, plant or part thereof comprises a polynucleotide as shown in SEQ ID NO:
- 1, 3, 5, 7, 9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, 35, 37, 39, 41, 43, or 45, or a variant or derivative thereof, wherein the polynucleotide encodes an mut-PPO polypeptide that is used as a selection marker, and wherein said transformed plant cell, plant tissue, plant or part thereof may comprise a further isolated polynucleotide;
- ii) contacting the transformed plant cell, plant tissue, plant or part thereof with at least one benzoxazinine-derivative compound;
- iii) determining whether the plant cell, plant tissue, plant or part thereof is affected by the inhibiting compound;
- and iv) identifying or selecting the transformed plant cell, plant tissue, plant or part thereof.
Dependencies are inferred from the claim text.