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US 2026/0117192 A1Published application
Methods of producing a tumor avatar
AssigneeAriel Scientific Innovations Ltd.
InventorsGabriela ROZIC, Igor KOMAN
Life of this patent
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Abstract
Three-dimensional tumor avatars comprising an artificial scaffold, tumor cells, tumor associated endothelial cells and tumor associated fibroblast cells are provided. Kits comprising the 3D tumor avatars, methods of producing the 3D tumor avatars and methods of using the 3D tumor avatars are also provided.
Claims21
1-53. (canceled)
A three-dimensional (3D) tumor avatar comprising:
- an artificial scaffold, tumor cells, and tumor microenvironmental (TME) cells comprising tumor associated endothelial cells (TECs) and cancer associated fibroblasts (CAFs).
A method for producing a 3D tumor avatar, the method comprising:
- a) providing tumor cells and tumor microenvironmental (TME) cells comprising tumor associated endothelial cells (TECs) and cancer associated fibroblasts (CAFs);
- a) culturing said tumor cells and said TME cells with an artificial scaffold in a liquid media suitable for in vitro organization and maintenance of said 3D tumor avatar;
- thereby producing a 3D tumor avatar.
The method of 67, wherein said culturing of step (b) is in liquid media comprising
- a. DMEM/F12, ECM:FM-2:MSCM (1:1:1) or DMEM/F12:ECM:MSCM:FM-2 (1-10:1:1:1);
- and
- b. Hepes, ABAM, 2 GlutaMax supplement, R-spondin 1, Noggin, Wnt3a, epidermal growth factor (EGF), Gastrin, fibroblast growth factor 2 (FGF2), FGF10, gastrin, prostaglandin E2, A83-01, nicotinamide, and Vascular endothelial growth factor (VEGF), interleukin-6 (IL-6), IL-8, Bone Morphogenetic Protein 4 (BMP4) and BMP7 to produce said organoids, and optionally Y-27632, SB202190, trombospondin and hydrocortisone.
Dependencies are inferred from the claim text.