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US 2026/0117154 A1Published application
Systems and Methods for Immobilizing Extracellular Matrix Material on Organ on Chip, Multilayer Microfluidics Microdevices, and Three-Dimensional Cell Culture Systems
AssigneeThe Trustees of the University of Pennsylvania
InventorsDongeun Huh, Mark Mondrinos, Cassidy Blundell, Jeongyun Seo
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Abstract
The presently disclosed subject matter provides an approach to address the needs for microscale control in shaping the spacial geometry and microarchitecture of 3D collagen hydrogels. For example, the disclosed subject matter provides for compositions, methods, and systems employing N-sulfosuccinimidyl-6-(4′-azido-2′-nitro-phenylamino)hexanoate (“sulfo-SANPAH”), to prevent detachment of the hydrogel from the anchoring substrate due to cell-mediated contraction.
Claims8
A method of fabricating a microengineered perfusable lumen sculpted from tissue comprising:
- a) fabricating a microchannel in a first body, said first body forming a substrate for tissue growth, wherein the microchannel is fabricated by bonding a second body to the first body;
- b) injecting the microchannel with a heterobifunctional crosslinker;
- c) curing the heterobifunctional crosslinker;
- d) replacing the second body with a third body to form a four-sided microchannel having three different sides treated with the heterobifunctional crosslinker;
- e) injecting an extracellular matrix (ECM) gel layer embedded with a contractile tissue into the microchannel;
- and
- f) allowing cell-mediated contractile forces to shape tissue geometry as the gel layer contracts, forming a semicircular conduit along a length of the microchannel.
4 more elements
Dependencies are inferred from the claim text.