Infused Particles

An infused bone grafting composition comprising bone particles taken from whole bone, wherein the bone particles are at least partially demineralized or at least partially decalcified;

• wherein the bone particles are infused with a supernatant of biologic material, a polyampholyte cryoprotectant, or both;
• and wherein the bone particles have an aspherical shape, and
• wherein the supernatant is derived from one or more of a fatty marrow and a cellular marrow,
• wherein the supernatant includes a mixture of biologic material having non-whole cellular components including vesicular components, cell fragments, cellular excretions, cellular derivatives, and extracellular components;
• whole cells;
• or a combinations of the non-whole cellular components and whole cells, wherein the mixture is compatible with biologic function;
• and
• wherein the polyampholyte cryoprotectant forms a three-dimensional infusion impregnating and coating externally enveloping each of the bone particles and each of the non-whole cellular components and whole cells.

An infused bone grafting composition, comprising a bone particle mixture taken from whole bone, the bone particle mixture comprising a quantity of bone particles of one or more bone particle types, wherein the bone particle types are fully decalcified or fully demineralized bone particles, at least partially decalcified or at least partially demineralized bone particles, er mineralized and calcified bone particles, wherein the bone particles have an aspherical shape;

• and a supernatant of biologic material, a polyampholyte cryoprotectant, or both,
• wherein the supernatant is derived from one or more of a fatty marrow and a cellular marrow,
• wherein the supernatant includes a mixture of biologic material having non-whole cellular components cell fragments, cellular excretions, cellular derivatives, and extracellular components, whole cells, or a combination of the non-whole cellular components and whole cells, wherein the mixture is compatible with biologic function,
• wherein the polyampholyte cryoprotectant forms a three-dimensional infusion that impregnates and externally envelops each of the bone particles and each of the non-whole cellular components and whole cells.