Primer-probe combination and kit for rapid broad-spectrum detection of mycoplasma, and use thereof

Abstract

The invention discloses a primer-probe combination and a kit for the rapid broad-spectrum detection of mycoplasma, and the use thereof. The primer-probe combination comprises 10 forward primers, 5 reverse primers and 4 detection probes, wherein the forward primers have the sequences as shown in SEQ ID NOs. 1-10, the reverse primers have the sequences as shown in SEQ ID NOs. 11-15, and the detection probes have the sequences as shown in SEQ ID NOs. 16-19. The kit comprises an internal control plasmid and an internal control probe. The primer-probe combination in the kit provides broad coverage of mycoplasma types, capable of detecting over 132 species and 410 strains, with a limit of detection of 0.1-0.5 copies/μl and a sensitivity below 10 CFU/ml. It also monitors potential PCR inhibition in samples, thereby preventing false-negative results.

Dependencies are inferred from the claim text.