Method for clinical urinary tract infection and bacteremia detection

A method for detecting urinary tract infections, comprising steps of:

• collecting a biological sample from a subject,
• wherein said biological sample comprises a urine sample, and
• wherein said subject is a human;
• preparing one or more dilute preparations of said biological sample,
• wherein at least one of said one or more dilute preparations comprises said biological sample diluted in sterile water,
• adding solid media from a biological media test (BMT) kit to said one or more dilute preparations comprising said biological sample in sterile water,
• wherein said BMT kit is an Aquagenx® CBT EC+TC MPN Kit;
• incubating said one or more dilute preparations with said solid media;
• analyzing said dilute preparations for indicia of bacterial presence to generate an assessment,
• wherein said one or more dilute preparations are compared to a diagnostic key for said BMT kit,
• wherein said diagnostic key for said BMT kit comprises a turbidity key that indicates a presence of bacteria, a color key that indicates a presence of coliform species, and a fluorescence key that indicates a presence of E. coli;
• and
• generating a diagnosis for said subject based on said assessment.

A method for detecting bacteremia, comprising:

• collecting a biological sample from a subject,
• wherein said biological sample comprises a blood sample,
• wherein said subject is a human;
• generating an inoculated mannitol salt broth (MSB) comprising said biological sample,
• wherein said inoculated MSB comprises 10 g/L tryptone peptone and 1 g/L yeast extract,
• wherein said inoculated MSB comprises neither 10 g/L proteose peptone nor 1 g/L beef extract;
• incubating said inoculated MSB;
• analyzing said inoculated MSB for indicia of bacterial presence to generate an assessment,
• wherein said inoculated MSB is compared to a diagnostic key for inoculated MSB,
• wherein said diagnostic key for inoculated MSB comprises a turbidity key that indicates a presence of Staphylococcus species and a color key that indicates a presence of Staphylococcus aureus;
• and
• generating a diagnosis for said subject based on said assessment.