Herbicide-resistant genes for mitochondrial transformation
AssigneeNapigen, Inc.
InventorsNarendra Singh YADAV, Mina HAMIDI, Hajime SAKAI, Elmer HEPPARD, Dilbag Singh MULTANI, Sachie KIMURA, Cheryl S. CASTER, Colleen MCMICHAEL, Emil Meyer OROZCO, Jr., Max Gabriel SCHUBERT, Ganesh Murthy KISHORE
Life of this patent
Estimating the term…
Abstract
The present disclosure relates to genetically modified cells containing mitochondria that have been transformed with a polynucleotide encoding an herbicide-resistant enzyme, such that the cells can grow in the presence of an inhibitor of the enzyme.
Claims21
-152. (canceled)
A method for transforming a mitochondrion of a cell, the method comprising:
- a) introducing a first polynucleotide encoding a first polypeptide into the mitochondrion, wherein the cell is a plant cell or an algal cell, wherein the first polypeptide is a variant of a naturally occurring polypeptide, wherein the naturally occurring polypeptide comprises an enzyme activity that is inhibited by an herbicide, wherein the variant of the naturally occurring polypeptide comprises an enzyme activity that is resistant to the herbicide;
- b) growing the cell under conditions wherein the first polypeptide is expressed;
- c) growing the cell in a medium wherein the herbicide is present at an effective concentration;
- and
- d) selecting a transformed cell comprising a transformed mitochondrion, wherein the transformed mitochondrion comprises the first polynucleotide.
A method for transforming a mitochondrion of a cell, the method comprising:
- a) introducing into the mitochondrion, wherein the cell is a plant cell or an algal cell:
- i) a first polynucleotide encoding a first polypeptide, wherein the first polypeptide is a variant of a naturally occurring polypeptide, wherein the naturally occurring polypeptide comprises an enzyme activity that is inhibited by an herbicide, wherein the variant of the naturally occurring polypeptide comprises an enzyme activity that is resistant to the herbicide, and
- ii) a second polynucleotide encoding a selectable marker, wherein the selectable marker enables the cell to grow in the presence of a selective agent, wherein the second polynucleotide does not encode the first polypeptide;
- b) growing the cell under conditions wherein the selectable marker is expressed;
- c) growing the cell in a medium comprising the selective agent, wherein the selective agent is present at an effective concentration;
- and
- d) selecting a transformed cell comprising a transformed mitochondrion, wherein the transformed mitochondrion comprises the first polynucleotide, and further wherein the transformed cell comprising the transformed mitochondrion is capable of growing in a medium comprising the herbicide when the herbicide is present at an effective concentration.
A method comprising growing a plurality of plants in a presence of an herbicide that is an inhibitor of a plant enzyme, wherein at least one plant of the plurality of plants comprises a mitochondrion comprising a heterologous polynucleotide that encodes a variant of the plant enzyme, wherein the variant of the plant enzyme has an enzyme activity resistant to the herbicide, wherein the presence of the herbicide is sufficient to selectively promote growth of the at least one plant of the plurality of plants, resulting in an increased growth of the at least one plant of the plurality of plants relative to plants lacking the heterologous polynucleotide.
Dependencies are inferred from the claim text.