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AssigneeBenson Hill, Inc.
InventorsMatthew Brett Begemann, Emma Elizabeth January, Erin Zess
Estimating the term…
Provided herein are plants, plant parts, a population of plants or plant parts, and plant products (e.g., seed composition, protein composition) comprising reduced activity of a protein-related polypeptide [e.g., stomatal cytokinesis defective 2 (SCD2), SCD2A, SCD2B, response to dehydration 22 (RD22), glucuronidase 3 (GUS3), GUS3A, glycosyl hydrolase family 10 protein B (GH10B), protein phosphatase 2A beta subunit (PP2AB), PPA2BA, PP2ABAB, alpha/beta-hydrolases superfamily protein (ABH), ABHA, ABHB, calmodulin-binding transcription activator protein 2 (CAMTA2), CAMTA2A, CAMTA2B, cinnamyl-alcohol dehydrogenase (CAD1), beta-ketoacyl reductase 1 (KCR1), KCR1A, or KCR1B], and compositions and methods of producing such plants and plant parts. The plants, plant parts, population of plants or plant parts, or plant products can have a genetic mutation that reduces the protein-related polypeptide activity, which can be one located at least partially in a protein-related gene (e.g., CAD1) or its homolog or in its regulatory region, and can have increased protein content and/or white flake protein content.
A plant or plant part comprising decreased activity of a protein-related polypeptide compared to a control plant or plant part, wherein said plant or plant part comprises a genetic mutation that decreases the activity of said protein-related polypeptide, and wherein said protein-related polypeptide is selected from the group consisting of cinnamyl-alcohol dehydrogenase 1 (CAD1), cinnamyl-alcohol dehydrogenase (CAD), stomatal cytokinesis defective 2 (SCD2), stomatal cytokinesis defective 2A (SCD2A), stomatal cytokinesis defective 2B (SCD2B), response to dehydration 22 (RD22), glucuronidase 3 (GUS3), glucuronidase 3A (GUS3A), glycosyl hydrolase family 10 protein B (GH10B), protein phosphatase 2A beta subunit (PP2AB), protein phosphatase 2A beta subunit A (PP2ABA), protein phosphatase 2A beta subunit B (PP2ABB), alpha/beta-hydrolases superfamily protein (ABH), alpha/beta-hydrolases superfamily protein A (ABHA), alpha/beta-hydrolases superfamily protein B (ABHB), calmodulin-binding transcription activator protein 2 (CAMTA2), calmodulin-binding transcription activator protein 2A (CAMTA2A), calmodulin-binding transcription activator protein 2B (CAMTA2B), beta-ketoacyl reductase 1 (KCR1), beta-ketoacyl reductase 1A (KCR1A), and beta-ketoacyl reductase 1B (KCR1B).
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A method for increasing protein content and/or white flake protein content in a plant or plant part, said method comprising reducing level or activity of at least one endogenous gene encoding a protein-related polypeptide in said plant or plant part, wherein said protein-related polypeptide is selected from the group consisting of cinnamyl-alcohol dehydrogenase 1 (CAD1), cinnamyl-alcohol dehydrogenase (CAD), stomatal cytokinesis defective 2 (SCD2), stomatal cytokinesis defective 2A (SCD2A), stomatal cytokinesis defective 2B (SCD2B), response to dehydration 22 (RD22), glucuronidase 3 (GUS3), glucuronidase 3A (GUS3A), glycosyl hydrolase family 10 protein B (GH10B), protein phosphatase 2A beta subunit (PP2AB), protein phosphatase 2A beta subunit A (PP2ABA), protein phosphatase 2A beta subunit B (PP2ABB), alpha/beta-hydrolases superfamily protein (ABH), alpha/beta-hydrolases superfamily protein A (ABHA), alpha/beta-hydrolases superfamily protein B (ABHB), calmodulin-binding transcription activator protein 2 (CAMTA2), calmodulin-binding transcription activator protein 2A (CAMTA2A), calmodulin-binding transcription activator protein 2B (CAMTA2B), beta-ketoacyl reductase 1 (KCR1), beta-ketoacyl reductase 1A (KCR1A), and beta-ketoacyl reductase 1B (KCR1B).
A method for increasing protein content and/or white flake protein content in a plant or plant part, said method comprising introducing a genetic mutation that decreases activity of a protein-related polypeptide into said plant or plant part, wherein said protein-related polypeptide is selected from the group consisting of cinnamyl-alcohol dehydrogenase 1 (CAD1), cinnamyl-alcohol dehydrogenase (CAD), stomatal cytokinesis defective 2 (SCD2), stomatal cytokinesis defective 2A (SCD2A), stomatal cytokinesis defective 2B (SCD2B), response to dehydration 22 (RD22), glucuronidase 3 (GUS3), glucuronidase 3A (GUS3A), glycosyl hydrolase family 10 protein B (GH10B), protein phosphatase 2A beta subunit (PP2AB), protein phosphatase 2A beta subunit A (PP2ABA), protein phosphatase 2A beta subunit B (PP2ABB), alpha/beta-hydrolases superfamily protein (ABH), alpha/beta-hydrolases superfamily protein A (ABHA), alpha/beta-hydrolases superfamily protein B (ABHB), calmodulin-binding transcription activator protein 2 (CAMTA2), calmodulin-binding transcription activator protein 2A (CAMTA2A), calmodulin-binding transcription activator protein 2B (CAMTA2B), beta-ketoacyl reductase 1 (KCR1), beta-ketoacyl reductase 1A (KCR1A), and beta-ketoacyl reductase 1B (KCR1B).
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A nucleic acid molecule comprising a nucleic acid sequence of a mutated protein-related gene or coding sequence thereof, wherein said nucleic acid sequence comprises any one of SEQ ID NOs:
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Dependencies are inferred from the claim text.