Self-replicating rna and use thereof

A replicable RNA molecule, comprising, from the 5′ end to the 3′ end, a 5′ cap, a 5′ UTR, an open reading frame encoding an RNA replicase, a promoter, a second 5′ UTR, a sequence of interest, a 3′ UTR and a poly(A) tail, wherein the RNA replicase is capable of amplifying the replicable RNA molecule and is capable of amplifying an RNA molecule containing the second 5′ UTR, the sequence of interest, and the 3′ UTR, wherein the RNA replicase is a nonstructural protein or a functional variant thereof of a self-replicating virus, wherein the second 5′ UTR comprises a nucleotide sequence set forth in SEQ ID NO:

• 5, 6, 7 or 8.

(canceled)

A replicable RNA molecule, comprising, from the 5′ end to the 3′ end, a 5′ cap, a 5′ UTR, an open reading frame encoding an RNA replicase, a promoter, a first sequence of interest, an internal ribosome entry site (IRES), a second sequence of interest, a 3′ UTR and a poly(A) tail, wherein the RNA replicase is capable of amplifying the replicable RNA molecule, and is capable of amplifying an RNA molecule containing the first sequence of interest, the internal ribosome entry site, the second sequence of interest, and the 3′ UTR, wherein the RNA replicase is a nonstructural protein or a functional variant thereof of a self-replicating virus, wherein one of the first and second sequences of interest is an open reading frame encoding an immunosuppressive protein, wherein a second 5′ UTR is further comprised between the promoter and the first sequence of interest, wherein the RNA replicase is capable of amplifying an RNA molecule containing the second 5′ UTR, the first sequence of interest, the internal ribosome entry site, the second sequence of interest, and the 3′ UTR, and wherein the second 5′ UTR comprises a nucleotide sequence set forth in SEQ ID NO:

• 5, 6, 7 or 8.

-29. (canceled)