System and Method For Converting Adipose Derived Mesenchymal Stem Cells To Hematopoietic Stem/Progenitor Cells And Differentiating Into Blood Cells And Applications Of Same
AssigneeUl Cell Therapeutic Pte. Ltd
InventorsWei LI, Shaojin YOU, Michael Chengnan LIN, Annie YOU
Life of this patent
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Abstract
A method of producing hematopoietic stem/progenitor cells (HS/PCs) and blood cells from adipose derived mesenchymal stem cells (Ad MSCs). The method comprises obtaining biological cells of a subject; expanding the biological cells; obtaining a pure Ad MSCs cell line from the expanded biological cells; converting the pure Ad MSCs cell line into HS/PCs in vitro; expanding the converted HS/PCs in vitro; and obtaining the expanded HS/PCs.
Claims25
A method of producing hematopoietic stem/progenitor cells (HS/PCs) and blood cells from adipose derived mesenchymal stem cells (Ad MSCs), the method comprising:
- obtaining biological cells of a subject;
- expanding the biological cells;
- obtaining a pure Ad MSCs cell line from the expanded biological cells;
- converting the pure Ad MSCs cell line into HS/PCs in vitro;
- expanding the converted HS/PCs in vitro;
- and
- obtaining the expanded HS/PCs.
A HS/PCs converting kit for converting adipose derived mesenchymal stem cells (Ad MSCs) into hematopoietic stem/progenitor cells (HS/PCs), the kit comprising:
- a basic Ad MSCs culture medium;
- a basic nutrition supplement;
- and
- a basic converting supplement mixture,
- wherein the kit converts the Ad MSCs into HS/PCs.
14-20. (canceled)
An automatic multifunctional single cell processor and culture device, the device comprising:
- a housing;
- at least one small fluid volume dispenser system disposed inside the housing;
- and
- a tissue grinding system disposed below the small fluid volume dispenser system and in the housing;
- wherein the small fluid volume dispenser system is configured to dispense at least one fluid;
- and wherein the tissue grinding system receives the at least one fluid.
Dependencies are inferred from the claim text.