Microglia derived from pluripotent stem cells and methods of making and using the same
OwnerPanagiotis Douvaras, Scott Noggle +2inventors
InventorsPanagiotis Douvaras, Scott Noggle, Stephen Chang, Valentina Fossati
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Abstract
The present invention provides methods and compositions for the generation of microglial progenitor cells and microglial cells from pluripotent stem cells, such as embryonic stem cells and induced pluripotent stem cells. The present invention also provides cells produced using such methods, and both methods of treatment and methods of drug screening that use such cells. Also provided are various tissue culture media, tissue culture media supplements, and kits useful for the generation of human microglial progenitor cells and human microglial cells.
Claims25
A method for generating microglial cells comprising:
- a) culturing human pluripotent stem cells under conditions that induce myeloid differentiation, leading to the generation of CD14+ and/or CX3CR1+ microglial progenitor cells, and
- b) culturing the CD14+ and/or CX3CR1+ microglial progenitor cells in either (i) a first microglial differentiation medium comprising IL-34, or (ii) a second microglial differentiation medium comprising M-CSF, thereby generating human microglial cells.
Dependencies are inferred from the claim text.