Engineered microorganisms with enhanced protein expression and secretion
AssigneeNorth Carolina State University
InventorsNathan Crook, Deniz Durmusoglu, Ibrahim Al'Abri, Daniel Haller
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Abstract
The present disclosure provides materials and methods related to the production of peptides and polypeptides from engineered microorganisms. In particular, the present disclosure provides compositions and methods for producing a genetically modified microorganism (e.g., yeast cell) with enhanced protein expression and/or secretion.
Claims24
An engineered yeast cell comprising:
- (i) at least one genetic modification that produces a functional phosphoglucomutase (PGM2) protein;
- and
- (ii) at least one exogenous polynucleotide operably linked to a pGAL1 promoter;
- wherein expression of a target polypeptide or protein encoded by the exogenous polynucleotide is increased compared to a yeast cell lacking the at least one genetic modification.
13-16. (canceled)
21-27. (canceled)
A method of growing a yeast cell in media comprising galactose, the method comprising:
- making at least one genetic modification to the yeast cell such that the yeast cells is capable of producing a functional phosphoglucomutase (PGM2) protein;
- wherein the yeast cell is a Saccharomyces boulardii cell;
- and
- wherein the at least one genetic modification enables the yeast cell to utilize galactose as a carbon source.
(canceled)
A method of enhancing colonization of a yeast cell in a host, the method comprising:
- administering a composition comprising an engineered yeast cell that comprises at least one genetic modification that produces a functional phosphoglucomutase (PGM2) protein;
- wherein the yeast cell is a Saccharomyces boulardii cell;
- and
- wherein the at least one genetic modification enables the yeast cell to utilize galactose as a carbon source.
33-35. (canceled)
Dependencies are inferred from the claim text.