Anti-crispr delivery compositions and methods
AssigneesThe Brigham and Women's Hospital, Inc., Massachusetts Institute of Technology
InventorsAmit Choudhary, Axel O. Vera, Bradley Pentelute, Nicholas Truex, Ronald T. Raines
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Abstract
Described in certain exemplary embodiments herein are engineered Anti-CRISPR (Acr) polypeptides and delivery systems engineered for delivery to the cytosol and/or nucleus of cells. In certain embodiments, an engineered Acr polypeptide comprises an Acr polypeptide operatively coupled to a cargo delivery molecule, wherein the cargo delivery molecule is capable of binding or otherwise interacting with a pore-forming polypeptide. Also described in certain exemplary embodiments are methods of Acr delivery to cells via the engineered Acr polypeptides and delivery systems of the present disclosure.
Claims31
An engineered Anti-CRISPR (Acr) polypeptide comprising:
- an Acr polypeptide operatively coupled to a cargo delivery molecule, wherein the cargo delivery molecule is capable of binding or otherwise interacting with a pore-forming polypeptide.
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Dependencies are inferred from the claim text.