Tri-segmented pichinde viruses as vaccine vectors
AssigneesHookipa Biotech GmbH, UniversitäT Basel
InventorsWeldi Bonilla, Daniel David Pinschewer, Klaus Orlinger
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Abstract
The present application relates to Pichinde viruses with rearrangements of their open reading frames (“ORF”) in their genomes. In particular, described herein is a modified Pichinde virus genomic segment, wherein the Pichinde virus genomic segment is engineered to carry a viral ORF in a position other than the wild-type position of the ORF. Also described herein are trisegmented Pichinde virus particles comprising one L segment and two S segments or two L segments and one S segment. The Pichinde virus, described herein may be suitable for vaccines and/or treatment of diseases and/or for the use in immunotherapies.
Claims21
-71. (canceled)
A tri-segmented Pichinde virus particle comprising one L segment and two S segments, wherein:
- the L segment comprises an open reading frame (ORF) encoding RNA-dependent RNA polymerase (L protein) under control of a Pichinde virus 3′ untranslated region (UTR) and an ORF encoding matrix protein Z (Z protein) under the control of a Pichinde virus 5′ UTR;
- the first S segment comprises an ORF encoding the Pichinde viral glycoprotein (GP) under the control of a Pichinde virus 3′ UTR and an ORF encoding a first antigen under the control of a Pichinde virus 5′ UTR;
- and
- the second S segment comprises an ORF encoding a nucleoprotein (NP) under the control of a Pichinde virus 3′ UTR and an ORF encoding a second antigen under the control of a Pichinde virus 5′ UTR.
Dependencies are inferred from the claim text.