Roller pump for use with a perfusion system and associated methods

A method of calibrating a roller pump within an extracorporeal perfusion system having a control unit, the method comprising:

• positioning a flexible tubing within a raceway of a roller pump, wherein the roller pump comprises:
• a roller pump rotor comprising a first roller arm having a first roller rotatably coupled thereto and a second roller arm having a second roller rotatably coupled thereto, wherein the raceway is disposed around the roller pump rotor;
• and
• an electronic actuator disposed on the roller pump rotor, wherein the electronic actuator is configured to change a radial position of the first roller and the second roller relative to a central axis of the roller pump rotor;
• activating the roller pump to rotate the roller pump rotor relative to the raceway with the first roller and the second roller disposed in a radially innermost position corresponding to zero flow within the flexible tubing;
• actuating the electronic actuator to move the first roller and the second roller radially outward from the radially innermost position until a flow rate greater than zero is measured within the flexible tubing;
• storing a lower flow limit within the control unit, the lower flow limit corresponding to a first radially outermost position at which the flow rate was measured at zero;
• actuating the electronic actuator to move the first roller and the second roller radially outward from the first radially outermost position until the flow rate measured within the flexible tubing stops changing;
• and
• storing an upper flow limit within the control unit, the upper flow limit corresponding to a second radially outermost position at which the flow rate measured within the flexible tubing stopped changing.