Phytic acid-based ternary complex biomimetic nanomaterial, and preparation method and use thereof

A phytic acid (PA)-based ternary complex biomimetic nanomaterial, wherein

• the biomimetic nanomaterial is a ternary complex composed of PA, a metal ion and a protein/polypeptide, wherein a molar ratio of the protein/polypeptide to the PA is 1:25;
• the ternary complex is formed by connecting the protein/polypeptide with the PA using a metal cation as a cation bridge;
• the metal ion is a multivalent metal cation;
• the protein/polypeptide is selected from the group consisting of common bovine serum albumin, human serum albumin, targeting protein transferrin, phycocyanin with photothermal properties, and targeting peptide Arg-Gly-Asp (RGD);
• the multivalent metal cation is selected from the group consisting of Cr3+, Co2+, Mn2+, Fe2+, Fe3+, Cu2+, Cu3+, La2+, Gd3+, Ce3+, Tb3+, Pr3+, Dy3+, Nd3+, Ho3+, Pm3+, Er3+, Sm3+, Tm3+, Eu3+, Yb3+, Lu3+, Bi3+, Hf4+, Re4+, W4+, Ta5+, 177Lu3+, 68Ga3+, 99mTc7+, 111In3+, 67Cu2+, Pt2+, Ru3+, Os2+, 223Ra2+, 89Sr2+, 60Co2+, 192Ir3+, 137Cs+, and 182Ta5+;
• after a selected metal ion solution is mixed with the PA solution at a molar ratio of phytic acid to metal ion of 1:(1-6), preparation method I or preparation method II is used provided that no precipitate is produced;
• and preparation method II is selected provided that a precipitate is produced;
• the preparation method I comprises steps of:
• S1-1:
• mixing PA and a metal ion solution at a molar ratio of PA to metal ion of 1:(1-6), and stirring until uniform to obtain mixed solution A;
• S1-2:
• adding a protein/polypeptide solution to the mixed solution A at a molar ratio of protein/polypeptide to PA of 1:25, and adjusting a pH value of a resulting solution to 7-10 to obtain mixed solution B;
• and
• S1-3:
• centrifuging mixed solution B, removing a resulting pellet, and subjecting a resulting supernatant to repeated ultrafiltration/dialysis to obtain a ternary complex solution, which is the biomimetic nanomaterial;
• the preparation method II comprises steps of:
• S2-1:
• providing a PA solution, adjusting a pH value of the PA solution to 7-10, adding a protein/polypeptide solution to the PA solution at a molar ratio of protein/polypeptide to PA of 1:25, and stirring until uniform to obtain mixed solution C;
• S2-2:
• adding a metal ion solution to the mixed solution C at a molar ratio of metal ion to PA of 3:1, and stirring until uniform to obtain mixed solution D, wherein the metal ion in the metal ion solution is a metal ion that produces a precipitate after mixed with the PA solution;
• and
• S2-3:
• centrifuging the mixed solution D, remove a resulting pellet, and subjecting a resulting supernatant to repeated ultrafiltration/dialysis to obtain a ternary complex solution, which is the biomimetic nanomaterial.