Regenerative compositions and methods of making and using the same
AssigneeINVO Aesthetics Inc.
InventorNathan CONNOLLY
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Abstract
Provided herein are novel compositions comprising Human Mesenchymal Conditioned Media from mammalian-derived, such as human-derived, cell sources, wherein the cell sources comprise bone marrow mesenchymal cells (BM-MSCs), umbilical cord mesenchymal cells (UC-MSCs), and fibroblast cells. The compositions may be used for formulations and skincare solutions suitable for use for cosmeceutical applications.
Claims23
A composition comprising Human Mesenchymal Conditioned Media from human-derived cell sources, wherein the human-derived cell sources comprise bone marrow mesenchymal cells (BM-MSCs), umbilical cord mesenchymal cells (UC-MSCs), and fibroblast cells,
- wherein the BM-MSCs, UC-MSCs, and fibroblast cells are cultured in growth media specifically formulated for optimal cell proliferation and optimized for the secretion of regenerative molecules,
- wherein the growth media for BM-MSCs comprises Dulbecco's Modified Eagle Medium-Ham's F-12 (DMEM-F12) supplemented with fetal bovine serum (FBS), and growth factors (rhFGF basic, IGF-1);
- wherein the growth factors for UC-MSCs comprises Dulbecco's Modified Eagle Medium-Ham's F-12 (DMEM-F12) supplemented with fetal bovine serum (FBS), and growth factors (rhEGF, rhFGF basic, rhFGF acidic);
- wherein the growth media for culturing fibroblast cells comprises Dulbecco's Modified Eagle Medium (DMEM) with 8% FBS.
A method for cosmetically improving skin conditions comprising:
- utilizing novel formulations consisting of Composition A, B, C or D Formulations,
- wherein the formulations comprise Human Mesenchymal Conditioned Media,
- wherein the Human Mesenchymal Conditioned Media is obtained from human-derived cell sources comprising BM-MSCs, UC-MSCs, and fibroblast cells,
- wherein the BM-MSCs, UC-MSCs, and fibroblast cells are cultured under unique conditions comprising the use of proprietary growth media,
- wherein the proprietary growth media is specifically formulated for optimal cell proliferation and optimized for the secretion of regenerative molecules, and
- monitoring the dermatological condition for improvement.
Dependencies are inferred from the claim text.