Method for evaluating influence by gene-edited low-gossypol cotton on aphis gossypii

A method for evaluating an influence by a gene-edited low-gossypol cotton on Aphis gossypii, comprising the following steps:

• S1, planting the gene-edited low-gossypol cotton indoors;
• S2, collecting a fully expanded young leaf from a top part of a seedling of the gene-edited low-gossypol cotton and placing the young leaf in a medium in a container, and then inoculating nymphal Aphis gossypii on the young leaf;
• S3, rearing the nymphal Aphis gossypii until the 6th day after it is developed into an adult Aphis gossypii and lay new nymphal Aphis gossypii, recording a number of dead adult Aphis gossypii and a number of newly laid nymphal Aphis gossypii every 24 h, and terminating the recording when all Aphis gossypii individuals die in an initial test;
• and
• S4, calculating an Aphis gossypii mortality rate and an average Aphis gossypii productivity, and evaluating the influence by the gene-edited low-gossypol cotton on the Aphis gossypii based on the Aphis gossypii mortality rate and the average Aphis gossypii productivity.