Methods for producing high protein soybeans

A method selecting plants in a segregating population comprising a high protein CCT allele, the method comprising:

• a. self-pollinating a first soybean plant or first soybean germplasm or crossing the first soybean plant or first soybean germplasm with a second soybean plant or second soybean germplasm to form a soybean population comprising a plurality of soybean plants or soybean germplasm, the soybean plants or soybean germplasm comprising a CCT gene and the soybean population comprising a high protein CCT allele of the CCT gene and a wild-type CCT allele of the CCT gene;
• b. isolating nucleic acids from the soybean plants or soybean germplasm of the population;
• c. assaying the one or more nucleic acids for the presence of the high protein CCT allele by detecting a nucleotide polymorphism in the CCT gene sequence having at least 95% identity to SEQ ID NO:
• 51;
• d. assaying the one or more nucleic acids for the presence of the wild-type CCT allele having at least 95% identity to SEQ ID NO:
• 51;
• and
• e. selecting from the plurality of soybean plants or soybean germplasm one or more soybean plants or soybean germplasm comprising two high protein CCT alleles or comprising one high protein CCT allele and one wild-type CCT allele, or a combination thereof.

A method for introgressing a high protein CCT domain containing variant sequence into a soybean plant or soybean germplasm, the method comprising:

• a. crossing a first soybean plant or first soybean germplasm with a second soybean plant or second soybean germplasm to form a soybean plant or soybean germplasm population, wherein the first soybean plant or soybean germplasm or the second soybean plant or germplasm comprises the high protein CCT domain containing variant sequence;
• b. isolating nucleic acids from the soybean plants or soybean germplasm of the population;
• c. assaying the one or more nucleic acids for the presence of the high protein CCT allele by detecting a nucleotide polymorphism in the CCT gene sequence having at least 95% identity to SEQ ID NO:
• 51;
• d. assaying the one or more nucleic acids for the presence of a wild-type CCT allele having at least 95% identity to SEQ ID NO:
• 51;
• and
• e. selecting from the plurality of soybean plants or soybean germplasm one or more soybean plants or soybean germplasm comprising at least one high protein CCT allele.