High speed scanning systems for super resolution imaging
AssigneePacific Biosciences of California, Inc.
InventorsWindsor OWENS, Bryan P. STAKER, Robert HARTLAGE, Edvinas ZIZMINSKAS, Paul HEILMAN, Jim JAHNCKE
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Abstract
Disclosed herein is a high throughput optical scanning system to generate super resolution images and methods of use. The optical scanning device and methods of use provided herein can allow high throughput scanning of a continuously moving object with a high resolution despite fluctuations in stage velocity. This can aid in high throughput scanning of a substrate, such as a biological chip comprising fluorophores. Also provided herein are improved optical relay systems and scanning optics.
Claims3
-37. (canceled)
An imaging system comprising:
- a light source;
- one or more imaging sensors operably coupled to an objective lens to capture at least a field of a plurality of fields of a substrate;
- a stage to support said substrate, wherein said stage moves along a path;
- an encoder operably coupled to said stage;
- a position processing module to receive positional information from said encoder and generate a plurality of calculated positions for said stage;
- and
- a scanning mirror coupled to an axis adjacent to said path to scan said field of said plurality of fields of said substrate,
- wherein said scan comprises a forward scan and a back scan, and wherein said forward scan and said back scan are generated by said position processing module based on said plurality of calculated positions.
A method of imaging a moving substrate:
- providing a substrate on a stage, wherein said substrate comprises a plurality of fields, and wherein an encoder is operably coupled to said stage;
- translating said stage along a path;
- determining a plurality of positions of said stage from a position processing module electrically coupled to said encoder, wherein said position processing module performs a plurality of calculations based on said plurality of positions of said stage;
- and
- actuating a scanning mirror coupled to an axis adjacent to said path to scan said light source with said scanning mirror along a field of said plurality of fields of said substrate, wherein said scan comprises a forward scan and a back scan generated by said position processing module based on said plurality of calculations.
Dependencies are inferred from the claim text.