Chemiluminescence-based dual flow rapid diagnostic testing platform and methods of use

A lateral flow assay for detecting a target in a liquid sample, the lateral flow assay comprising:

• a first strip defining a first flow path, the first strip comprising:
• a first sample pad;
• a chemiluminescent substrate pad adjacent to and in fluid communication with the first sample pad, the chemiluminescent substrate pad comprising a dried or lyophilized chemiluminescent substrate, wherein the dried or lyophilized chemiluminescent substrate forms a reconstituted chemiluminescent substrate when contacted with the liquid sample;
• a delay pad adjacent to and in fluid communication with the chemiluminescent substrate pad, the delay pad is structured and arranged to have a reduced flow velocity;
• and
• a membrane that generates a signal of immunoassay adjacent to and in fluid communication with the delay pad comprising immobilized capture antibodies;
• and
• a second strip partially overlapping the first strip and defining a second flow path, the second strip comprising:
• a second sample pad at least partially overlapping and in fluid communication with the first sample pad;
• and
• a conjugate pad adjacent to and in fluid communication with the second sample pad, the conjugate pad having an end extending to partially overlap and be in fluid communication with the membrane that generates a signal of immunoassay of the first strip at which point the first flow path and the second flow path merge, and the conjugate pad having a dried or lyophilized detection antibody conjugated with an enzyme, wherein the dried or lyophilized detection antibody conjugated with an enzyme forms a reconstituted detection antibody conjugated with an enzyme when contacted with the liquid sample;
• wherein:
• the first sample pad and the second sample pad are structured and arranged to receive a liquid sample through absorption, and the liquid sample separately flows through the first flow path and the second flow path;
• and
• the lateral flow assay is structured and arranged wherein the liquid sample flowing through the second flow path flows through the membrane that generates a signal of immunoassay for generating an immunoassay signal before the liquid sample flowing through the first flow path.