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US 2026/0103738 A1Published application
Cell culture compositions and methods for polypeptide production
AssigneeGenentech, Inc.
InventorsSteven J. Meier, Melissa S. Mun, Natarajan Vijayasankaran, Sharat Varma, Yi Yang, Boyan Zhang, Silvana R. Arevalo, Martin Gawlitzek, Veronica Carvalhal
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Abstract
Cell culture media, such as chemically defined cell culture media, are provided, as are methods of using the media for cell growth (i.e., cell culture) and polypeptide (e.g., antibody) production. Compositions comprising polypeptides produced by the methods are also provided.
Claims15
A method of culturing Chines Hamster Ovary (CHO) cells to produce E25, an anti-IgE antibody, comprising:
- (a) culturing a CHO cell expressing the E25 antibody in a cell culture medium comprising:
- (i) from about 200 mg/L to about 1200 mg/L cystine;
- (ii) from about 2 μM to about 80 μM ferric citrate;
- (iii) from about 0.05 mg/L to about 1.0 mg/L vitamin B2;
- (iv) from about 0.05 mg/L to about 10.0 mg/L vitamin B6;
- (v) from about 0.05 mg/L to about 12.0 mg/L vitamin B9;
- (vi) from about 0.05 mg/L to about 2.5 mg/L vitamin B12;
- (b) purifying the E25 antibody from the cell culture;
- and
- (c) concentrating the purified E25 antibody to at least a 100 mg/mL E25 antibody solution, wherein the E25 antibody solution has a color reference standard value selected from the group consisting of B4-B9, BY4-BY7, Y4-Y7, GY4-GY7 and R4-R7 according to the European Pharmacopoeia color standards, European Pharmacopoeia, 2008 7th Edition, Page 22.
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Dependencies are inferred from the claim text.