Microorganisms producing low linoleic acid oils and methods of use

A microorganism comprising one or more genetic modifications, wherein the genetic modification comprise at least one of the following genetic modifications:

• a. a MNT1 deletion, under-production, under-expression, inactivation, or negative attenuation;
• a deletion, under-production, under-expression, inactivation, or negative attenuation of a MNT1 homologue;
• a deletion, under-production, under-expression, inactivation, or negative attenuation of a protein comprising an amino acid sequence set forth in SEQ ID NO. 27, SEQ ID NO. 72, or SEQ ID NO. 73;
• a deletion, under-production, under-expression, inactivation, or negative attenuation of a protein comprising an amino acid sequence which is at least 70%, 75%, 80%, 85%, 90%, or 95% similar to SEQ ID NO. 27, SEQ ID NO. 72, or SEQ ID NO. 73;
• or a deletion, under-production, under-expression, inactivation, or negative attenuation of a protein which catalyzes a reaction defined by EC 2.4.1;
• b. an AGS1 deletion, under-production, under-expression, inactivation, or negative attenuation;
• a deletion, under-production, under-expression, inactivation, or negative attenuation of an AGS1 homologue;
• a deletion, under-production, under-expression, inactivation, or negative attenuation of a protein comprising an amino acid sequence set forth in SEQ ID NO. 28, SEQ ID NO. 74, SEQ ID NO. 75, or SEQ ID NO. 76;
• a deletion, under-production, under-expression, inactivation, or negative attenuation of a protein comprising an amino acid sequence which is at least 70%, 75%, 80%, 85%, 90%, or 95% similar to SEQ ID NO. 28;
• or a deletion, under-production, under-expression, inactivation, or negative attenuation of a protein which catalyzes a reaction defined by EC 2.4.1.183;
• c. a VEL1 overexpression, overproduction, activation, or positive attenuation;
• an overexpression, overproduction, activation, or positive attenuation of a VEL1 homologue;
• an overexpression, overproduction, activation, or positive attenuation of a protein comprising an amino acid sequence set forth in SEQ ID NO. 59, SEQ ID NO. 77, SEQ ID NO. 78, SEQ ID NO. 79, or SEQ ID NO. 80;
• or an overexpression, overproduction, activation, or positive attenuation of a protein comprising an amino acid sequence which is at least 70%, 75%, 80%, 85%, 90%, or 95% similar SEQ ID NO. 59, SEQ ID NO. 77, SEQ ID NO. 78, SEQ ID NO. 79, or SEQ ID NO. 80;
• or
• d. a VEL4 overexpression, overproduction, activation, or positive attenuation;
• an overexpression, overproduction, activation, or positive attenuation of a VEL4 homologue;
• an overexpression, overproduction, activation, or positive attenuation of a protein comprising an amino acid sequence set forth in SEQ ID NO. 58, or SEQ ID NO. 81, SEQ ID NO. 82, SEQ ID NO. 83, or SEQ ID NO. 84;
• or an overexpression, overproduction, activation, or positive attenuation of a protein comprising an amino acid sequence which is at least 70%, 75%, 80%, 85%, 90%, or 95% similar SEQ ID NO. 58, or SEQ ID NO. 81, SEQ ID NO. 82, SEQ ID NO. 83, or SEQ ID NO. 84.