Vector for multi-gene base editing, editing method, and use

Abstract

Provided are a vector for multi-gene base editing, an editing method, and a use, and belongs to the technical field of gene editing. This application provides a vector for multi-gene base editing. In the vector, a replicon element, U6, and sgRNA are linked in series to allow a plasmid carrying the replicon element to persist in a cell, such that other elements on the plasmid can be continuously expressed in the cell, which improves the efficiency of multi-gene silencing. This application also provides a tandem vector, which is obtained by removing an epi element from the vector above. The two multi-gene editing vectors constructed in the embodiments of the present disclosure both have a silencing editing effect for target loci. The vector including the epi element has a higher editing efficiency than the vector without the epi element.

Dependencies are inferred from the claim text.