US 2026/0103719 A1 · POLYNUCLEOTIDE, PROTEIN, BIOLOGICAL MATERIAL, AND USE THEREOF IN IMPROVING QUALITY OF PLANT FRUIT

Polynucleotide, protein, biological material, and use thereof in improving quality of plant fruit

AssigneesAgricultural Genomics Institute, Chinese Academy of Agricultural Sciences, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Agricultural Genomics Institute At Shenzhen, Chinese Academy of Agricultural Sciences

InventorsSanwen HUANG, Jinzhe ZHANG, Hongjun LYU, Jie CHEN, Guangtao ZHU

Life of this patent

Estimating the term…

Abstract

Disclosed are a polynucleotide, a protein, a biological material, and use thereof in the improvement of the quality of a plant fruit. The polynucleotide includes any one of the sequences set forth in SEQ ID NOs: 3, 11, 23-36, and 95-108; a complementary sequence, a degenerate sequence, or a homologous sequence thereof; a sequence hybridized with the sequence or a complementary sequence thereof under rigorous conditions; and a cDNA sequence of the sequence. It is found that the gene is a key regulatory gene affecting the sugar content of the fruit, and in the use, the content of SSC, glucose, fructose, citric acid, and malic acid of the plant fruit can be regulated by regulating the protein function, the expression level, and the like of CDPK gene without significantly affecting the weight of the fruit, such that the quality and flavor of a tomato fruit are improved.

Claims18

Dependent claims

A plant or part thereof, wherein CDPK8 gene and/or CDPK9 gene of the plant or part thereof are knocked out;

wherein a polynucleotide of CDPK8 gene is at least one of the following nucleotide sequences:
(1) a sequence having at least 90% sequence identity to any one of the sequences set forth in SEQ ID NOs:
3 and 23-36;
and
(2) a sequence encoding a protein comprising a sequence having at least 90% sequence identity and the same function as any one of the sequences set forth in SEQ ID NOs:
2 and 66-94;
and
a polynucleotide of CDPK9 gene is at least one of the following nucleotide sequences:
(1) a sequence having at least 90% sequence identity to any one of the sequences set forth in SEQ ID NOs:
11 and 95-108;
and
(2) a sequence encoding a protein comprising a sequence having at least 90% sequence identity and the same function as any one of the sequences set forth in SEQ ID NOs:
10 and 153-196.

10 more elements

A method for improving a quality of a plant fruit, wherein a phosphorylation level of SuSy3 protein is reduced by at least one of the following methods:

S1. inhibiting an expression of CDPK or inactivating a phosphorylase of CDPK;
a polynucleotide of CDPK gene is a polynucleotide of CDPK8 gene and/or CDPK9 gene, a polynucleotide of CDPK8 gene is at least one of the following nucleotide sequences:
(1) a sequence having at least 90% sequence identity to any one of the sequences set forth in SEQ ID NOs:
3 and 23-36;
and
(2) a sequence encoding a protein comprising a sequence having at least 90% sequence identity and the same function as any one of the sequences set forth in SEQ ID NOs:
2 and 66-94;
and
a polynucleotide of the CDPK9 gene is at least one of the following nucleotide sequences:
(1) a sequence having at least 90% sequence identity to any one of the sequences set forth in SEQ ID NOs:
11 and 95-108;
and
(2) a sequence encoding a protein comprising a sequence having at least 90% sequence identity and the same function as any one of the sequences set forth in SEQ ID NOs:
10 and 153-196;
or
an amino acid sequence of CDPK protein is at least one of the following sequences:
(21) a sequence as set forth in any one of SEQ ID NOs:
2 and 66-94;
(22) a fusion protein obtained by connecting a tag to N-terminus and/or C-terminus of any of the sequences set forth in SEQ ID NOs:
2 and 66-94;
(23) a protein having the same function obtained by substituting and/or deleting and/or adding one or more amino acid residues in any of the sequences set forth in SEQ ID NOs:
2 and 66-94;
(24) a protein having at least 90% identity to any one of the sequences set forth in SEQ ID NOs:
2 and 66-94 and having the same function;
(25) a sequence as set forth in any one of SEQ ID NOs:
10 and 153-196;
(26) a fusion protein obtained by connecting a tag to the N-terminus and/or C-terminus of any of the sequences set forth in SEQ ID NOs:
10, and 153-196;
(27) a protein having the same function obtained by substituting and/or deleting and/or adding one or more amino acid residues in any of the sequences set forth in SEQ ID NOs:
10 and 153-196;
and
(28) a protein having at least 90% identity with the sequence set forth in any one of SEQ ID NOs:
10 and 153-196 and having the same function;
S2. mutating one or more amino acids in SuSy3 protein, wherein a mutation site is a phosphorylation site mediated by CDPK, without altering the glycosyltransferase activity of the SuSy3 protein;
or
S3. specifically overexpressing the SuSy3 protein in the fruit.

33 more elements

Dependencies are inferred from the claim text.

Facts

Application

19/234,630Filed 11 Jun 2025

Priority

CN 202211616622.815 Dec 2022

Published

16 Apr 2026

Term ends

Not estimatedThe source records don’t support an estimate.

Classification19 codes

C12N 15/8245
Involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine: involving modified carbohydrate or sugar…
A01H 5/00
Angiosperms, i.e. flowering plants, characterised by their plant parts
A01H 6/82
Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy: solanaceae, e.g. pepper, tobacco, potato, tomato or eggplant
C07K 14/415
Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof: from plants