Methods and compositions for selective regulation of protein expression
OwnerCara L. GRIFFITH, Jeongwoon KIM +2inventors
InventorsCara L. GRIFFITH, Jeongwoon KIM, Brent A. O'BRIEN, Heping YANG
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Abstract
The invention provides novel recombinant DNA, compositions, and methods for selectively regulating the expression of a transcribable polynucleotide or recombinant protein in pollen of a transgenic plant. The invention also provides transgenic plants, plant cells, plant parts, seeds, and commodity products comprising such recombinant DNA polynucleotides and compositions.
Claims20
A recombinant DNA polynucleotide comprising an mts-siRNA target element comprising a DNA sequence selected from the group consisting of:
- i. a sequence with at least 90 percent sequence identity to SEQ ID NO:1;
- and
- ii. a sequence comprising SEQ ID NO:1;
- wherein the mts-siRNA target element is operably linked to a heterologous transcribable DNA polynucleotide.
A transgenic plant cell comprising a recombinant DNA polynucleotide comprising an mts-siRNA target element comprising a DNA sequence selected from the group consisting of:
- i. a sequence with at least 90 percent sequence identity to SEQ ID NO:1;
- and
- ii. a sequence comprising SEQ ID NO:1;
- wherein the mts-siRNA target element is operably linked to a heterologous transcribable DNA polynucleotide.
A method of reducing expression of a transcribable DNA polynucleotide in male reproductive tissues of a plant comprising the steps:
- i. operably linking an mts-siRNA target element to said transcribable DNA polynucleotide;
- wherein said mts-siRNA target element is heterologous with respect to the transcribable DNA polynucleotide;
- and wherein the mts-siRNA target element has at least 90 percent sequence identity to SEQ ID NO:1;
- ii. transforming a plant with an expression cassette comprising the transcribable DNA polynucleotide operably linked to the mts-siRNA target element.
Dependencies are inferred from the claim text.