US 2026/0103693 A1Published application

Lipase variant

AssigneeKao Corporation

InventorsTakahiro HIOKI, Akihito KAWAHARA

Life of this patent

Estimating the term…

Abstract

Provided is a lipase mutant with improved heterologous expression. The lipase mutant consists of an amino acid sequence obtained by substituting an amino acid residue at a predetermined position numbered according to SEQ ID NO: 2 with a predetermined amino acid residue in an amino acid sequence of a parent lipase.

Claims15

Dependent claims

A lipase mutant selected from the group consisting of (a) to (v) and (aa) to (ae):

(a) a lipase mutant consisting of an amino acid sequence having an identity of at least 82% with the amino acid sequence of SEQ ID NO:
2 and having aspartic acid at a position corresponding to position 118 of the amino acid sequence of SEQ ID NO:
2;
(b) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
4 and having tyrosine at a position corresponding to position 31 of the amino acid sequence of SEQ ID NO:
2;
(c) a lipase mutant consisting of an amino acid sequence having an identity of at least 91% with the amino acid sequence of SEQ ID NO:
4 and having glycine at a position corresponding to position 32 of the amino acid sequence of SEQ ID NO:
2;
(d) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
4 and having cysteine at a position corresponding to position 90 of the amino acid sequence of SEQ ID NO:
2;
(e) a lipase mutant consisting of an amino acid sequence having an identity of at least 94% with the amino acid sequence of SEQ ID NO:
4 and having leucine at a position corresponding to position 181 of the amino acid sequence of SEQ ID NO:
2;
(f) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
6 and having alanine, threonine, histidine, or glycine at a position corresponding to position 222 of the amino acid sequence of SEQ ID NO:
2;
(g) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
8 and having lysine at a position corresponding to position 39 of the amino acid sequence of SEQ ID NO:
2;
(h) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
8 and having threonine at a position corresponding to position 125 of the amino acid sequence of SEQ ID NO:
2;
(i) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
8 and having proline at a position corresponding to position 126 of the amino acid sequence of SEQ ID NO:
2;
(j) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
8 and having glycine at a position corresponding to position 293 of the amino acid sequence of SEQ ID NO:
2;
(k) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
10 and having tyrosine at a position corresponding to position 29 of the amino acid sequence of SEQ ID NO:
2;
(l) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
10 and having tyrosine at a position corresponding to position 31 of the amino acid sequence of SEQ ID NO:
2;
(m) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
10 and having glycine at a position corresponding to position 32 of the amino acid sequence of SEQ ID NO:
2;
(n) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
10 and having glycine at a position corresponding to position 186 of the amino acid sequence of SEQ ID NO:
2;
(o) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
12 and having glutamine at a position corresponding to position 206 of the amino acid sequence of SEQ ID NO:
2;
(p) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
14 and having tyrosine at a position corresponding to position 31 of the amino acid sequence of SEQ ID NO:
2;
(q) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
14 and having glycine at a position corresponding to position 32 of the amino acid sequence of SEQ ID NO:
2;
(r) a lipase mutant consisting of an amino acid sequence having an identity of at least 97% with the amino acid sequence of SEQ ID NO:
14 and having lysine at a position corresponding to position 77 of the amino acid sequence of SEQ ID NO:
2;
(s) a lipase mutant consisting of an amino acid sequence having an identity of at least 83% with the amino acid sequence of SEQ ID NO:
14 and having leucine at a position corresponding to position 181 of the amino acid sequence of SEQ ID NO:
2;
(t) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
16 and having cysteine at a position corresponding to position 90 of the amino acid sequence of SEQ ID NO:
2;
(u) a lipase mutant consisting of an amino acid sequence having an identity of at least 81% with the amino acid sequence of SEQ ID NO:
16 and having leucine at a position corresponding to position 181 of the amino acid sequence of SEQ ID NO:
2;
(v) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
34 and having tyrosine at a position corresponding to position 29 of the amino acid sequence of SEQ ID NO:
2;
(aa) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
4 and having at least two amino acid residues selected from tyrosine at a position corresponding to position 31, glycine at a position corresponding to position 32, cysteine at a position corresponding to position 90, and leucine at a position corresponding to position 181 of the amino acid sequence of SEQ ID NO:
2;
(ab) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
8 and having at least two amino acid residues selected from lysine at a position corresponding to position 39, threonine at a position corresponding to position 125, proline at a position corresponding to position 126 and glycine at a position corresponding to position 293 of the amino acid sequence of SEQ ID NO:
2;
(ac) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
10 and having at least two amino acid residues selected from tyrosine at a position corresponding to position 29, tyrosine at a position corresponding to position 31, glycine at a position corresponding to position 32, and glycine at a position corresponding to position 186 of the amino acid sequence of SEQ ID NO:
2;
(ad) a lipase mutant consisting of an amino acid sequence having an identity of at least 83% with the amino acid sequence of SEQ ID NO:
14 and having at least two amino acid residues selected from tyrosine at a position corresponding to position 31, glycine at a position corresponding to position 32, lysine at a position corresponding to position 77, and leucine at a position corresponding to position 181 of the amino acid sequence of SEQ ID NO:
2;
and
(ae) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
16 and having cysteine at a position corresponding to position 90 and leucine at a position corresponding to position 181 of the amino acid sequence of SEO ID NO:
2.

79 more elements

A method for manufacturing a lipase mutant, comprising a step selected from the group consisting of (i) to (xxvii):

(i) a step of substituting an amino acid residue at a position corresponding to position 118 of the amino acid sequence of SEQ ID NO:
2 with aspartic acid in a polypeptide that consists of an amino acid sequence having an identity of at least 82% with the amino acid sequence of SEQ ID NO:
2 and has lipase activity;
(ii) a step of substituting an amino acid residue at a position corresponding to position 31 of the amino acid sequence of SEQ ID NO:
2 with tyrosine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
4 and has lipase activity;
(iii) a step of substituting an amino acid residue at a position corresponding to position 32 of the amino acid sequence of SEQ ID NO:
2 with glycine in a polypeptide that consists of an amino acid sequence having an identity of at least 91% with the amino acid sequence of SEQ ID NO:
4 and has lipase activity;
(iv) a step of substituting an amino acid residue at a position corresponding to position 90 of the amino acid sequence of SEQ ID NO:
2 with cysteine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
4 and has lipase activity;
(v) a step of substituting an amino acid residue at a position corresponding to position 181 of the amino acid sequence of SEQ ID NO:
2 with leucine in a polypeptide that consists of an amino acid sequence having an identity of at least 94% with the amino acid sequence of SEQ ID NO:
4 and has lipase activity;
(vi) a step of substituting an amino acid residue at a position corresponding to position 222 of the amino acid sequence of SEQ ID NO:
2 with alanine, threonine, histidine, or glycine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
6 and has lipase activity;
(vii) a step of substituting an amino acid residue at a position corresponding to position 39 of the amino acid sequence of SEQ ID NO:
2 with lysine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
8 and has lipase activity;
(viii) a step of substituting an amino acid residue at a position corresponding to position 125 of the amino acid sequence of SEQ ID NO:
2 with threonine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
8 and has lipase activity;
(ix) a step of substituting an amino acid residue at a position corresponding to position 126 of the amino acid sequence of SEQ ID NO:
2 with proline in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
8 and has lipase activity;
(x) a step of substituting an amino acid residue at a position corresponding to position 293 of the amino acid sequence of SEQ ID NO:
2 with glycine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
8 and has lipase activity;
(xi) a step of substituting an amino acid residue at a position corresponding to position 29 of the amino acid sequence of SEQ ID NO:
2 with tyrosine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
10 and has lipase activity;
(xii) a step of substituting an amino acid residue at a position corresponding to position 31 of the amino acid sequence of SEQ ID NO:
2 with tyrosine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
10 and has lipase activity;
(xiii) a step of substituting an amino acid residue at a position corresponding to position 32 of the amino acid sequence of SEQ ID NO:
2 with glycine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
10 and has lipase activity;
(xiv) a step of substituting an amino acid residue at a position corresponding to position 186 of the amino acid sequence of SEQ ID NO:
2 with glycine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
10 and has lipase activity;
(xv) a step of substituting an amino acid residue at a position corresponding to position 206 of the amino acid sequence of SEQ ID NO:
2 with glutamine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
12 and has lipase activity;
(xvi) a step of substituting an amino acid residue at a position corresponding to position 31 of the amino acid sequence of SEQ ID NO:
2 with tyrosine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
14 and has lipase activity;
(xvii) a step of substituting an amino acid residue at a position corresponding to position 32 of the amino acid sequence of SEQ ID NO:
2 with glycine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
14 and has lipase activity;
(xviii) a step of substituting an amino acid residue at a position corresponding to position 77 of the amino acid sequence of SEQ ID NO:
2 with lysine in a polypeptide that consists of an amino acid sequence having an identity of at least 97% with the amino acid sequence of SEQ ID NO:
14 and has lipase activity;
(xix) a step of substituting an amino acid residue at a position corresponding to position 181 of the amino acid sequence of SEQ ID NO:
2 with leucine in a polypeptide that consists of an amino acid sequence having an identity of at least 83% with the amino acid sequence of SEQ ID NO:
14 and has lipase activity;
(xx) a step of substituting an amino acid residue at a position corresponding to position 90 of the amino acid sequence of SEQ ID NO:
2 with cysteine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
16 and has lipase activity;
(xxi) a step of substituting an amino acid residue at a position corresponding to position 181 of the amino acid sequence of SEQ ID NO:
2 with leucine in a polypeptide that consists of an amino acid sequence having an identity of at least 81% with the amino acid sequence of SEQ ID NO:
16 and has lipase activity;
(xxii) a step of substituting an amino acid residue at a position corresponding to position 29 of the amino acid sequence of SEQ ID NO. 2 with tyrosine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
34 and has lipase activity;
(xxiii) a step of substituting at least two amino acid residues at positions selected from positions corresponding to positions 31, 32, 90, and 181 of the amino acid sequence of SEQ ID NO:
2 with tyrosine at a position corresponding to position 31, glycine at a position corresponding to position 32, cysteine at a position corresponding to position 90, and leucine at a position corresponding to position 181 in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
4 and has lipase activity;
(xxiv) a step of substituting at least two amino acid residues at positions selected from positions corresponding to positions 39, 125, 126, and 293 of the amino acid sequence of SEQ ID NO:
2 with lysine at a position corresponding to position 39, threonine at a position corresponding to position 125, proline at a position corresponding to position 126, and glycine at a position corresponding to position 293 in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
8 and has lipase activity;
(xxv) a step of substituting at least two amino acid residues at positions selected from positions corresponding to positions 29, 31, 32, and 186 of the amino acid sequence of SEQ ID NO:
2 with tyrosine at a position corresponding to position 29, tyrosine at a position corresponding to position 31, glycine at a position corresponding to position 32, and glycine at a position corresponding to position 186 in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
10 and has lipase activity;
(xxvi) a step of substituting at least two amino acid residues at positions selected from positions corresponding to positions 31, 32, 77, and 181 of the amino acid sequence of SEQ ID NO:
2 with tyrosine at a position corresponding to position 31, glycine at a position corresponding to position 32, lysine at a position corresponding to position 77, and leucine at a position corresponding to position 181 in a polypeptide that consists of an amino acid sequence having an identity of at least 83% with the amino acid sequence of SEQ ID NO:
14 and has lipase activity;
and
(xxvii) a step of substituting an amino acid residue at a position corresponding to position 90 of the amino acid sequence of SEQ ID NO:
2 with cysteine and an amino acid residue at a position corresponding to position 181 with leucine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
16 and has lipase activity.

78 more elements

A method for improving heterologous expression of a lipase, comprising a step selected from the group consisting of (i) to (xxvii):

(i) a step of substituting an amino acid residue at a position corresponding to position 118 of the amino acid sequence of SEQ ID NO:
2 with aspartic acid in a polypeptide that consists of an amino acid sequence having an identity of at least 82% with the amino acid sequence of SEQ ID NO:
2 and has lipase activity;
(ii) a step of substituting an amino acid residue at a position corresponding to position 31 of the amino acid sequence of SEQ ID NO:
2 with tyrosine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
4 and has lipase activity;
(iii) a step of substituting an amino acid residue at a position corresponding to position 32 of the amino acid sequence of SEQ ID NO:
2 with glycine in a polypeptide that consists of an amino acid sequence having an identity of at least 91% with the amino acid sequence of SEQ ID NO:
4 and has lipase activity;
(iv) a step of substituting an amino acid residue at a position corresponding to position 90 of the amino acid sequence of SEQ ID NO:
2 with cysteine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
4 and has lipase activity;
(v) a step of substituting an amino acid residue at a position corresponding to position 181 of the amino acid sequence of SEQ ID NO:
2 with leucine in a polypeptide that consists of an amino acid sequence having an identity of at least 94% with the amino acid sequence of SEQ ID NO:
4 and has lipase activity;
(vi) a step of substituting an amino acid residue at a position corresponding to position 222 of the amino acid sequence of SEQ ID NO:
2 with alanine, threonine, histidine, or glycine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
6 and has lipase activity;
(vii) a step of substituting an amino acid residue at a position corresponding to position 39 of the amino acid sequence of SEQ ID NO:
2 with lysine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
8 and has lipase activity;
(viii) a step of substituting an amino acid residue at a position corresponding to position 125 of the amino acid sequence of SEQ ID NO:
2 with threonine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
8 and has lipase activity;
(ix) a step of substituting an amino acid residue at a position corresponding to position 126 of the amino acid sequence of SEQ ID NO:
2 with proline in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
8 and has lipase activity;
(x) a step of substituting an amino acid residue at a position corresponding to position 293 of the amino acid sequence of SEQ ID NO:
2 with glycine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
8 and has lipase activity;
(xi) a step of substituting an amino acid residue in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
10 and has lipase activity with tyrosine at a position corresponding to position 29 of the amino acid sequence of SEQ ID NO:
2;
(xii) a step of substituting an amino acid residue at a position corresponding to position 31 of the amino acid sequence of SEQ ID NO:
2 with tyrosine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
10 and has lipase activity;
(xiii) a step of substituting an amino acid residue at a position corresponding to position 32 of the amino acid sequence of SEQ ID NO:
2 with glycine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
10 and has lipase activity:
(xiv) a step of substituting an amino acid residue at a position corresponding to position 186 of the amino acid sequence of SEQ ID NO:
2 with glycine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
10 and has lipase activity;
(xv) a step of substituting an amino acid residue at a position corresponding to position 206 of the amino acid sequence of SEQ ID NO:
2 with glutamine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
12 and has lipase activity;
(xvi) a step of substituting an amino acid residue at a position corresponding to position 31 of the amino acid sequence of SEQ ID NO:
2 with tyrosine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
14 and has lipase activity;
(xvii) a step of substituting an amino acid residue at a position corresponding to position 32 of the amino acid sequence of SEQ ID NO:
2 with glycine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
14 and has lipase activity;
(xviii) a step of substituting an amino acid residue at a position corresponding to position 77 of the amino acid sequence of SEQ ID NO:
2 with lysine in a polypeptide that consists of an amino acid sequence having an identity of at least 97% with the amino acid sequence of SEQ ID NO:
14 and has lipase activity;
(xix) a step of substituting an amino acid residue at a position corresponding to position 181 of the amino acid sequence of SEQ ID NO:
2 with leucine in a polypeptide that consists of an amino acid sequence having an identity of at least 83% with the amino acid sequence of SEQ ID NO:
14 and has lipase activity:
(xx) a step of substituting an amino acid residue at a position corresponding to position 90 of the amino acid sequence of SEQ ID NO:
2 with cysteine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
16 and has lipase activity;
(xxi) a step of substituting an amino acid residue at a position corresponding to position 181 of the amino acid sequence of SEQ ID NO:
2 with leucine in a polypeptide that consists of an amino acid sequence having an identity of at least 81% with the amino acid sequence of SEQ ID NO:
16 and has lipase activity;
(xxii) a step of substituting an amino acid residue at a position corresponding to position 29 of the amino acid sequence of SEQ ID NO:
2 with tyrosine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
34 and has lipase activity;
(xxiii) a step of substituting at least two amino acid residues at positions selected from positions corresponding to positions 31, 32, 90, and 181 of the amino acid sequence of SEQ ID NO:
2 with tyrosine at a position corresponding to position 31, glycine at a position corresponding to position 32, cysteine at a position corresponding to position 90, and leucine at a position corresponding to position 181 in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
4 and has lipase activity;
(xxiv) a step of substituting at least two amino acid residues at positions selected from positions corresponding to positions 39, 125, 126, and 293 of the amino acid sequence of SEQ ID NO:
2 with lysine at a position corresponding to position 39, threonine at a position corresponding to position 125, proline at a position corresponding to position 126, and glycine at a position corresponding to position 293 in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
8 and has lipase activity;
(xxv) a step of substituting at least two amino acid residues at positions selected from positions corresponding to positions 29, 31, 32, and 186 of the amino acid sequence of SEQ ID NO:
2 with tyrosine at a position corresponding to position 29, tyrosine at a position corresponding to position 31, glycine at a position corresponding to position 32, and glycine at a position corresponding to position 186 in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
10 and has lipase activity;
(xxvi) a step of substituting at least two amino acid residues at positions selected from positions corresponding to positions 31, 32, 77, and 181 of the amino acid sequence of SEQ ID NO:
2 with tyrosine at a position corresponding to position 31, glycine at a position corresponding to position 32, lysine at a position corresponding to position 77, and leucine at a position corresponding to position 181 in a polypeptide that consists of an amino acid sequence having an identity of at least 83% with the amino acid sequence of SEQ ID NO:
14 and has lipase activity;
and
(xxvii) a step of substituting an amino acid residue at a position corresponding to position 90 of the amino acid sequence of SEQ ID NO:
2 with cysteine and an amino acid residue at a position corresponding to position 181 with leucine in a polypeptide that consists of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
16 and has lipase activity.

79 more elements

Dependencies are inferred from the claim text.

Facts

Application: 19/113,016Filed 19 Sep 2023
Priority: JP 2022-14938220 Sep 2022
Published: 16 Apr 2026
Term ends: Not estimatedThe source records don’t support an estimate.

Classification10 codes

C12N 9/20
Carboxylic ester hydrolases: triglyceride splitting, e.g. by means of lipase
C12N 15/75
Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora: for Bacillus
C12Y 301/01003
Carboxylic ester hydrolases: triacylglycerol lipase
C12R 2001/01
Microorganisms ; Processes using microorganisms: bacteria or Actinomycetales ; using bacteria or Actinomycetales

Family

No related US applications are recorded in the indexed filing history.

Timeline

Citations

Open elsewhere

patentgrep

Loading patent…

Abstract

A lipase mutant selected from the group consisting of (a) to (v) and (aa) to (ae):

(a) a lipase mutant consisting of an amino acid sequence having an identity of at least 82% with the amino acid sequence of SEQ ID NO:
2 and having aspartic acid at a position corresponding to position 118 of the amino acid sequence of SEQ ID NO:
2;
(b) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
4 and having tyrosine at a position corresponding to position 31 of the amino acid sequence of SEQ ID NO:
2;
(c) a lipase mutant consisting of an amino acid sequence having an identity of at least 91% with the amino acid sequence of SEQ ID NO:
4 and having glycine at a position corresponding to position 32 of the amino acid sequence of SEQ ID NO:
2;
(d) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
4 and having cysteine at a position corresponding to position 90 of the amino acid sequence of SEQ ID NO:
2;
(e) a lipase mutant consisting of an amino acid sequence having an identity of at least 94% with the amino acid sequence of SEQ ID NO:
4 and having leucine at a position corresponding to position 181 of the amino acid sequence of SEQ ID NO:
2;
(f) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
6 and having alanine, threonine, histidine, or glycine at a position corresponding to position 222 of the amino acid sequence of SEQ ID NO:
2;
(g) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
8 and having lysine at a position corresponding to position 39 of the amino acid sequence of SEQ ID NO:
2;
(h) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
8 and having threonine at a position corresponding to position 125 of the amino acid sequence of SEQ ID NO:
2;
(i) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
8 and having proline at a position corresponding to position 126 of the amino acid sequence of SEQ ID NO:
2;
(j) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
8 and having glycine at a position corresponding to position 293 of the amino acid sequence of SEQ ID NO:
2;
(k) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
10 and having tyrosine at a position corresponding to position 29 of the amino acid sequence of SEQ ID NO:
2;
(l) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
10 and having tyrosine at a position corresponding to position 31 of the amino acid sequence of SEQ ID NO:
2;
(m) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
10 and having glycine at a position corresponding to position 32 of the amino acid sequence of SEQ ID NO:
2;
(n) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
10 and having glycine at a position corresponding to position 186 of the amino acid sequence of SEQ ID NO:
2;
(o) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
12 and having glutamine at a position corresponding to position 206 of the amino acid sequence of SEQ ID NO:
2;
(p) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
14 and having tyrosine at a position corresponding to position 31 of the amino acid sequence of SEQ ID NO:
2;
(q) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
14 and having glycine at a position corresponding to position 32 of the amino acid sequence of SEQ ID NO:
2;
(r) a lipase mutant consisting of an amino acid sequence having an identity of at least 97% with the amino acid sequence of SEQ ID NO:
14 and having lysine at a position corresponding to position 77 of the amino acid sequence of SEQ ID NO:
2;
(s) a lipase mutant consisting of an amino acid sequence having an identity of at least 83% with the amino acid sequence of SEQ ID NO:
14 and having leucine at a position corresponding to position 181 of the amino acid sequence of SEQ ID NO:
2;
(t) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
16 and having cysteine at a position corresponding to position 90 of the amino acid sequence of SEQ ID NO:
2;
(u) a lipase mutant consisting of an amino acid sequence having an identity of at least 81% with the amino acid sequence of SEQ ID NO:
16 and having leucine at a position corresponding to position 181 of the amino acid sequence of SEQ ID NO:
2;
(v) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
34 and having tyrosine at a position corresponding to position 29 of the amino acid sequence of SEQ ID NO:
2;
(aa) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
4 and having at least two amino acid residues selected from tyrosine at a position corresponding to position 31, glycine at a position corresponding to position 32, cysteine at a position corresponding to position 90, and leucine at a position corresponding to position 181 of the amino acid sequence of SEQ ID NO:
2;
(ab) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
8 and having at least two amino acid residues selected from lysine at a position corresponding to position 39, threonine at a position corresponding to position 125, proline at a position corresponding to position 126 and glycine at a position corresponding to position 293 of the amino acid sequence of SEQ ID NO:
2;
(ac) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
10 and having at least two amino acid residues selected from tyrosine at a position corresponding to position 29, tyrosine at a position corresponding to position 31, glycine at a position corresponding to position 32, and glycine at a position corresponding to position 186 of the amino acid sequence of SEQ ID NO:
2;
(ad) a lipase mutant consisting of an amino acid sequence having an identity of at least 83% with the amino acid sequence of SEQ ID NO:
14 and having at least two amino acid residues selected from tyrosine at a position corresponding to position 31, glycine at a position corresponding to position 32, lysine at a position corresponding to position 77, and leucine at a position corresponding to position 181 of the amino acid sequence of SEQ ID NO:
2;
and
(ae) a lipase mutant consisting of an amino acid sequence having an identity of at least 80% with the amino acid sequence of SEQ ID NO:
16 and having cysteine at a position corresponding to position 90 and leucine at a position corresponding to position 181 of the amino acid sequence of SEO ID NO:
2.

79 more elements

Facts

Application

19/113,016Filed 19 Sep 2023

Priority

JP 2022-14938220 Sep 2022

Published

16 Apr 2026

Term ends

Not estimatedThe source records don’t support an estimate.

Classification10 codes

C12N 9/20
Carboxylic ester hydrolases: triglyceride splitting, e.g. by means of lipase
C12N 15/75
Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora: for Bacillus
C12Y 301/01003
Carboxylic ester hydrolases: triacylglycerol lipase
C12R 2001/01
Microorganisms ; Processes using microorganisms: bacteria or Actinomycetales ; using bacteria or Actinomycetales