Esterase mutants having polyester degradation activity and use thereof

Esterase mutants having polyester degradation activity, wherein the esterase mutants are one of the following:

• MT-1:
• glutamic acid at the 177th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into glutamine;
• MT-2:
• asparagine at the 178th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into alanine;
• MT-3:
• serine at the 180th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into threonine;
• MT-4:
• serine at the 180th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into leucine;
• MT-5:
• serine at the 180th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into valine;
• MT-6:
• isoleucine at the 181st site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into valine;
• MT-7:
• glycine at the 208th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into alanine;
• MT-8:
• serine at the 209th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into threonine;
• MT-9:
• serine at the 211th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into tyrosine;
• MT-10:
• glutamic acid at the 177th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into glutamine and serine at the 180th site is mutated into leucine;
• MT-11:
• glutamic acid at the 177th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into glutamine and isoleucine at the 181st site is mutated into valine;
• MT-12:
• glutamic acid at the 177th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into glutamine and glycine at the 208th site is mutated into alanine;
• MT-13:
• asparagine at the 178th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into alanine and glutamic acid at the 177th site is mutated into glutamine;
• MT-14:
• asparagine at the 178th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into alanine and serine at the 180th site is mutated into leucine;
• MT-15:
• asparagine at the 178th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into alanine and isoleucine at the 181st site is mutated into valine;
• MT-16:
• asparagine at the 178th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into alanine and glycine at the 208th site is mutated into alanine;
• MT-17:
• asparagine at the 178th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into alanine and serine at the 209th site is mutated into threonine;
• MT-18:
• serine at the 180th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into threonine and glutamic acid at the 177th site is mutated into glutamine;
• MT-19:
• serine at the 180th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into threonine and asparagine at the 178th site is mutated into alanine;
• MT-20:
• serine at the 180th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into threonine and isoleucine at the 181st site is mutated into valine;
• MT-21:
• serine at the 180th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into threonine and glycine at the 208th site is mutated into alanine;
• MT-22:
• serine at the 180th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into threonine and serine at the 209th site is mutated into threonine;
• MT-23:
• serine at the 180th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into threonine and serine at the 211th site is mutated into tyrosine;
• MT-24:
• serine at the 180th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into leucine and isoleucine at the 181st site is mutated into valine;
• MT-25:
• serine at the 180th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into leucine and glycine at the 208th site is mutated into alanine;
• MT-26:
• serine at the 180th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into valine and glutamic acid at the 177th site is mutated into glutamine;
• MT-27:
• serine at the 180th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into valine and asparagine at the 178th site is mutated into alanine;
• MT-28:
• serine at the 180th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into valine and isoleucine at the 181st site is mutated into valine;
• MT-29:
• serine at the 180th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into valine and glycine at the 208th site is mutated into alanine;
• MT-30:
• serine at the 180th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into valine and serine at the 209th site is mutated into threonine;
• MT-31:
• isoleucine at the 181st site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into valine and glycine at the 208th site is mutated into alanine;
• MT-32:
• serine at the 209th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into threonine and glutamic acid at the 177th site is mutated into glutamine;
• MT-33:
• serine at the 209th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into threonine and serine at the 180th site is mutated into leucine;
• MT-34:
• serine at the 209th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into threonine and isoleucine at the 181st site is mutated into valine;
• MT-35:
• serine at the 209th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into threonine and glycine at the 208th site is mutated into alanine;
• MT-36:
• serine at the 211th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into tyrosine and glutamic acid at the 177th site is mutated into glutamine;
• MT-37:
• serine at the 211th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into tyrosine and asparagine at the 178th site is mutated into alanine;
• MT-38:
• serine at the 211th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into tyrosine and serine at the 180th site is mutated into leucine;
• MT-39:
• serine at the 211th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into tyrosine and serine at the 180th site is mutated into valine;
• MT-40:
• serine at the 211th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into tyrosine and isoleucine at the 181st site is mutated into valine;
• MT-41:
• serine at the 211th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into tyrosine and glycine at the 208th site is mutated into alanine;
• and
• MT-42:
• serine at the 211th site of the amino acid sequence of an esterase A as shown in SEQ ID NO. 3 is mutated into tyrosine and serine at the 209th site is mutated into threonine.