Preparation method for corn superoxide dismutase

A preparation method for a corn superoxide dismutase, comprising following steps:

• S1. pre-treatment:
• pulverizing sprouted corn to 80-120 mesh, into the pulverized sprouted corn, adding a pH=7.8, 0.05 mol/L phosphate-buffered saline at a ratio of 1:2-4, and mixing uniformly and grinding to obtain a corn slurry, followed by ultrasonic cell disruption;
• S2. enzymatic hydrolysis:
• S21. transferring the corn slurry having undergone ultrasonic disruption into a rotary screen, and immersing a lower half of a drum into an enzymatic hydrolysis mixed liquid;
• starting the rotary screen, so as to enable a corn material to tumble inside the drum while remaining immersed in the enzymatic hydrolysis mixed liquid;
• S22. extracting the enzymatic hydrolysis mixed liquid every 10-15 minutes to undergo centrifugal separation, so as to remove suspended starch granules and other impurities;
• S23. pouring a centrifuged clear liquid back into the rotary screen, and performing enzymatic hydrolysis cyclically for 2-3 times;
• and
• S24. uniformly stirring the corn slurry having undergone the enzymatic hydrolysis and filtering, collecting a filtrate, and into the filtrate, adding an antioxidant of 0.5%-1.5% of a weight of the filtrate;
• and
• S3. extraction and post-treatment:
• S31. sonicating the filtrate to obtain a liquid, and adding any one or more of an activator, a protective agent and a stabilizer into the liquid;
• and
• S32. concentrating and drying:
• putting the filtrate in a low-temperature vacuum concentrator, and performing evaporation at 22-25° C. for 8-15 hours, so as to obtain a corn superoxide dismutase multienzyme liquid;
• and uniformly mixing the corn superoxide dismutase multienzyme liquid with silica accounting for 1% of a total weight, placing the mixture into a low-temperature vacuum drying device, and freeze-drying the same, so as to obtain a corn superoxide dismutase multienzyme powder.