Genetically modified vero cells
AssigneeWashington University
InventorsSiyuan Ding, Qiru Zeng, Yinxing Zhu
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Abstract
Among the various aspects of the present disclosure is the provision of genetically modified Vero cells. The present teachings include compositions for a rotavirus reverse genetics system that can include SERPINB1 knockout cells combined with a helper plasmid. The present teachings also include a vaccine-producing cell substrate that can include Vero cells with disrupted TMEM236 and method of use thereof.
Claims17
A rotavirus reverse genetics system, the system comprising a SERPINB1 knockout cell or a TMEM236 knockout cell.
A vaccine-producing cell substrate, the substrate comprising a Vero cell with disrupted SERPINB1 or TMEM236.
A method of increasing production of a vaccine in a cell, the method comprising:
- a. disrupting an anti-viral protein in the vaccine producing cell;
- b. introducing a helper component to the vaccine producing cell;
- c. infecting the vaccine producing cells with a live-attenuated vaccine;
- and
- d. collecting the vaccine from the vaccine producing cell.
Dependencies are inferred from the claim text.